HPLC法同时测定远志中2种活性成分的含量  

HPLC simultaneous determination of two active components in the roots of Polygala tenuifolia Willd

作　　者：刘艳芳[1] 杨晓娟[1] 田昕[1] 姜勇[1] 屠鹏飞[1] 

机构地区：[1]北京大学药学院天然药物与仿生药物国家重点实验室,北京100191

出　　处：《药物分析杂志》2010年第5期806-809,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金项目(30873429);"十一五"科技攻关项目(2006BAI09B05);2010年版中国药典修订项目(YZ-027;028);教育部新世纪优秀人才支持计划(985-2-102-113)

摘　　要：目的:建立同时测定中药远志中有效成分远志酮Ⅲ和3,6’-二芥子酰基蔗糖的HPLC含量测定方法。方法:采用Kromasil C18(250mm×4.6mm,5μm)色谱柱,以乙腈-0.05%磷酸水溶液(18:82)为流动相,流速1.0mL.min-1,检测波长320nm,柱温30℃。结果:远志酮Ⅲ及3,6’-二芥子酰基蔗糖的线性范围分别为0.5～4.0μg(r=0.9996)和0.5～8.0μg(r=0.9996),其平均回收率(n=6)分别为97.1%(RSD=0.87%)和99.4%(RSD=1.5%)。结论:该方法准确,重复性好,能够同时对远志中2种有效成分进行含量测定,为远志药材的质量控制提供了有效的方法。Objective：To develop an HPLC method for simultaneous determination of the two active ingredients,named polygalaxanthone Ⅲ and 3,6’-disinapoyl sucrose in the roots of Polygala tenuifolia.Methods：The marker compounds of polygalaxanthone Ⅲ and 3,6’-disinapoyl sucrose were separated on a Kromasil C18 column（250 mm×4.6 mm,5 μm）,the mobile phase was acetonitrile-0.05% phosphoric acid aqueous solution（18：82）at a flow rate of 1.0 mL·min-1,the detection wavelength was set at 320 nm and the column temperature was 30 ℃.Results：The assay was linear in the ranges of 0.5-4.0 μg（r=0.9996）for polygalaxanthone Ⅲ and 0.5-8.0 μg（r=0.9996）for 3,6’-disinapoyl sucrose.The average sample recoveries（n=6）were 97.1%（RSD=0.87%）and 99.4%（RSD=1.5%）for these two constituents,respectively.Conclusion：This accurate and reproducible method realizes the simultaneous determination of the two components in P.tenuifolia,which supplies an effective method for the quality control of P.tenuifolia.

关 键 词：远志 远志酮Ⅲ 3 6’-二芥子酰基蔗糖 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]