RP-HPLC法同时测定旱芹中绿原酸和咖啡酸的含量  

RP-HPLC simultaneous determination of chlorogenic acid and caffeic acid in Apium graveolens L

作　　者：杨洋[1] 谭亮[1] 陈晓辉[1] 毕开顺[1] 贾英[2] 

机构地区：[1]沈阳药科大学药学院,沈阳110016 [2]沈阳药科大学中药学院,沈阳110016

出　　处：《药物分析杂志》2010年第5期819-822,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立RP-HPLC法同时测定不同药用部位、不同产地、不同季节的旱芹中绿原酸和咖啡酸的含量。方法:采用Kromasil C18柱(5μm,250mm×4.6mm),流动相为甲醇-0.05%磷酸水溶液梯度洗脱,流速1.0mL.min-1,检测波长327nm,柱温35℃。结果:绿原酸和咖啡酸进样浓度分别在3.71～92.8μg.mL-1(r=0.9999)和0.148～3.70μg.mL-1(r=0.9997)范围内与峰面积呈良好的线性关系,平均加样回收率(n=9)分别为100.3%和100.2%。结论:分析方法快速、准确,重现性好,可用于控制旱芹的质量。Objective：To establish an RP-HPLC method for the simultaneous determination of chlorogenic acid and caffeic acid in Apium graveolens L.of different parts,different habitats and different seasons.Methods：The chromatographic method was carried out on Kromasil C18 column（5 μm,250 mm×4.6 mm）and methanol-0.05% phosphoric acid with gradient elution as the mobile phase was adopted;The flow rate was 1.0 mL min-1;The absorbance was monitored at 327 nm,and the column temperature was 35 ℃.Results：The linear response ranges were 3.71-92.8 μg·mL-1（r=0.9999）and 0.148-3.70 μg·mL-1（r=0.9997）,respectively.The average recoveries（n=9）were 100.3% and 100.2%,respectively.Conclusion：The assay demonstrates that the method has adequate accuracy and selectivity to measure the concentration of chlorogenic acid and caffeic acid in Apium graveolens L.

关 键 词：旱芹 不同部位 不同产地 不同季节 绿原酸 咖啡酸 

分 类 号：R917[医药卫生—药物分析学]