HPLC法测定华山参滴丸中消旋山莨菪碱、东莨菪碱及阿托品的含量  

HPLC analysis of raceanisodamine,scopolamine and atropine in Huashanshen dripping pills

作　　者：周军[1] 苗淑森 胡永峰[3] 曲佳[1] 吕曙华[1] 

机构地区：[1]天津市药品检验所,天津300070 [2]天津中新药业集团研究中心,天津300457 [3]天津中新药业集团有限公司第六中药厂,天津300401

出　　处：《药物分析杂志》2010年第5期838-841,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立HPLC法同时测定华山参滴丸中消旋山莨菪碱、东莨菪碱及阿托品的含量。方法:采用资生堂Capcell-Pak C18(4.6mm×250mm,5μm)色谱柱,以乙腈-醋酸盐缓冲液(5:95)为流动相A,乙腈-醋酸盐缓冲液(15:85)为流动相B,进行梯度洗脱(0～30min,60%A→35%A;30～40min,35%A→30%A;40～44min,30%A→20%A;44～45min,20%A→60%A;45～60min,60%A),流速1.0mL.min-1,检测波长210nm;柱温30℃。结果:消旋山莨菪碱、东莨菪碱及阿托品的线性范围分别为0.08～1.82,0.07～1.65,0.04～1.03μg(r=0.9999);平均回收率分别为99.1%(RSD=1.8%),97.0%(RSD=1.6%),104.0%(RSD=0.84%)。结论:所建立的HPLC法可同时测定华山参滴丸中消旋山莨菪碱、东莨菪碱及阿托品的含量。Objective：To develop an HPLC method for determination of raceanisodamine,scopolamine and atropine in Huashanshen dripping pills.Methods：The analysis was carried out on a Capcell-Pak C18（4.6 mm×250 mm,5 μm）column;The mobile phase was composed of acetonitrile-acetate buffer solution（5：95）（A）and acetonitrile-acetate buffer solution（15：85）（B）with gradient elution（0-30 min,60% A→35% A;30-40 min,35% A→30% A;40-44 min,30% A→20% A;44-45 min,20% A→60% A;45-60 min,60% A）and the flow rate was 1.0 mL·min-1;The detection wavelength was 210 nm,and the column temperature was 30 ℃.Results：The linear ranges of raceanisodamine,scopolamine and atropine were 0.08-1.82,0.07-1.65,0.04-1.03 μg（r=0.9999）,respectively;The average recoveries（n=6）were 99.1%（RSD=1.8%）,97.0%（RSD=1.6%）,104.0%（RSD=0.84%）,respectively.Conclusion：This method is simple and can be used to determine the above three components with satisfactory accuracy and repeatability.

关 键 词：高效液相色谱法 华山参滴丸 消旋山莨菪碱 东莨菪碱 阿托品 

分 类 号：R917[医药卫生—药物分析学]