RP-HPLC法同时测定芝参正肝胶囊中丹酚酸B、葛根素和芍药苷的含量  

RP-HPLC simultaneous determination of salvianolic acid B,puerarin and paeoniflorin in Zhishenzhenggan capsules

作　　者：叶晓平 茅仁刚[1,2] 袁萍[1,2] 

机构地区：[1]上海新康制药厂,上海201418 [2]上海师范大学,上海200234

出　　处：《药物分析杂志》2010年第5期887-890,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：上海市教委重点项目(编号CL200126);上海市科委优秀学科带头人计划(B类)(编号07XD14214)

摘　　要：目的:建立同时测定芝参正肝胶囊中丹酚酸B、葛根素和芍药苷含量的HPLC法。方法:采用Phenomenex Gemini C18(250mm×4.6mm,5μm)色谱柱,流动相为乙腈(A)-0.05mol.L-1磷酸二氢钾溶液(B),梯度洗脱,流速1.0mL.min-1,检测波长230nm,柱温30℃。结果:丹酚酸B、葛根素和芍药苷的线性范围分别为0.15～2.26μg(r=0.9999),0.10～1.53μg(r=0.9999),0.20～3.06μg(r=0.9999);平均加样回收率(n=9)分别为98.85%,99.15%,99.51%。结论:本法操作简便,结果可靠,适用于芝参正肝胶囊的质量控制。Objective：To propose an HPLC method for simultaneous determination of salvianolic acid B,puerarin and paeoniflorin in Zhishenzhenggan capsules.Methods：RP-HPLC analysis was carried out on a Phenomenex Gemini C18 column（250 mm×4.6 mm,5 μm）,mobile phase was binary solvent system of acetonitrile-0.05 mol·L-1 potassium dihydrogen at the flow rate of 1.0 mL·min-1,the detection wavelength was 230 nm,and column temperature was 30 ℃.Results：The linear ranges of salvianolic acid B,puerarin and paeoniflorin were 0.15-2.26 μg（r=0.9999）,0.10-1.53 μg（r=0.9999） and 0.20-3.06 μg（r=0.9999）,respectively;The average recoveries（n=9） of salvianolic acid B,puerarin and paeoniflorin were 98.85%,99.15% and 99.51% in Zhishenzhenggan capsules,respectively.Conclusion：The method is simple,reliable,and can be used for quantitative control of Zhishenzhenggan capsules.

关 键 词：芝参正肝胶囊 丹酚酸B 葛根素 芍药苷 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]