HPLC法梯度洗脱测定普鲁卡因青霉素中有关物质  

Determination of related substances in procaine benzylpenicillin by gradient elution of HPLC

作　　者：殷果[1] 秦斌[1] 邓颖[1] 王玉[1] 

机构地区：[1]深圳市药品检验所,深圳518029

出　　处：《药物分析杂志》2010年第5期925-928,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立梯度洗脱的HPLC法测定普鲁卡因青霉素中有关物质。方法:使用C18色谱柱,以缓冲液(pH7.0)-水-乙腈为流动相,梯度洗脱,流速1.0mL.min-1,检测波长225nm;采用青霉素杂质对照品配制系统适用性溶液。结果:本法可有效分离对氨基苯甲酸、普鲁卡因、各青霉素杂质、青霉素。结论:方法灵敏、准确,专属性强。Objective：To establish a method for the determination of related substances in procaine benzylpenicillin by using gradient elution of HPLC.Methods：C18 column was adopted;Mobile phase consisted of buffer （pH 7.0）-water-acetonitrile with gradient elution at a flow rate of 1.0 mL·min-1,and the detection wavelength was 225 nm.Results：4-Aminobenzoic acid,procaine,some impurities of benzylpenicillin and benzylpenicillin were obviously separated under the chromatographic system decribed above.Conclusion：The method is sensitive,accurate and specific.

关 键 词：高效液相色谱法 梯度洗脱 普鲁卡因 青霉素 有关物质 

分 类 号：R917[医药卫生—药物分析学]