奶牛FcRn受体α链基因启动子区的SSCP多态性研究  

SSCP Polymorphism Research of Promoter of FcRn α Chain Gene in Holstein Dairy Cows

作　　者：张春林[1,2] 王加启[1] 赵圣国[1] 赵国琦[2] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193

出　　处：《中国畜牧兽医》2010年第5期108-111,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30871837);国际科技合作项目(2009DFB30530);动物营养学国家重点实验室自主研究课题(2004DA125184(青)0801)

摘　　要：以189头荷斯坦奶牛为研究对象,对FcRn受体α链基因(FCGRT)启动区采用PCR-SSCP方法进行多态性检测。结果表明,FCGRT基因启动区在P1、P2和P3引物扩增片段中存在PCR-SSCP多态性,位于引物P1扩增产物有AA、AB、BB3种基因型,其频率分别为25.40%、59.26%、15.34%;引物P2扩增产物有CC、CD、DD3种基因型,其频率分别为20.63%、56.61%、22.75%;引物P3扩增产物有EE、EF、FF3种基因型,其频率分别为1.59%、12.17%、86.24%。经克隆测序分析,在3个片段上分别发生了T→C、C→A、G→T的碱基序列突变。经χ2适合性检验,除SNP1外,荷斯坦奶牛在该基因位点上的SNP2和SNP3均处于Hardy-Weinberg平衡状态。The PCR-SSCP polymorphisms of promoter of Fc receptor （FcRn） α chain （FCGRT） gene were studied in 189 Holstein cows. The results showed that PCR-SSCP polymorphisms were identified in promoter amplified fragment （P1,P2 and P3） of FCGRT gene. For the amplified fragment of P1,the genotype frequency of AA,AB and BB was 25.40%,59.26% and 15.34%,respectively. For the amplified fragment of P2,the genotype frequency of CC,CD and DD was 20.63%,56.61% and 22.75%,respectively. For the amplified fragment of P3,the genotype frequency of EE,EF and FF was 1.59%,12.17% and 86.24%,respectively.The sequencing analysis indicated that the polymorphisms were separately due to three single point mutation C to T,C to A and T to A at amplified fragment. Except for SNP1 in Holstein cows,the SNP2 and SNP3 sites in the populations were in a state of Hardy-Weinberg equlibrium.

关 键 词：荷斯坦奶牛 FCGRT启动区 PCR-SSCP 

分 类 号：Q78[生物学—分子生物学]