鹅甲状腺素应答蛋白α基因外显子2的多态性研究  

Polymorphism Analysis on Exon 2 of THRSPα Gene in Goose

作　　者：乔娜[1] 赵荣雪[1] 徐琪[1] 程金花[1] 赵文明[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009

出　　处：《中国畜牧兽医》2010年第5期112-115,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划重点项目(2006BDA14B06);现代农业产业技术体系建设专项资金(nycytx-45-04);优质高产水禽新品种选育国家科技部支撑计划重大专项资金(2006BDA01A09);江苏省属高校自然科学基础研究面上项目(07KJB230138)

摘　　要：为了解鹅THRSPα基因多态性,根据鸭THRSPα基因序列,设计引物扩增籽鹅、皖西白鹅、浙东白鹅和狮头鹅THRSPα基因外显子2,运用PCR-SSCP技术进行单核苷酸多态性分析。结果共检测到3个等位基因,6种基因型。测序结果表明,鹅THRSPα基因外显子2存在6个SNP位点。各品种中等位基因A占绝对优势。卡方检验结果表明,4个鹅群体均处于Hardy-Weinberg平衡状态(P>0.05)。试验结果证实,鹅THRSPα基因第2外显子存在序列多态性。This experiment was conducted to study the polymorphism on exon 2 of THRSPα gene in goose. The single nucleotide polymorphism （SNP） of thyroid hormone responsive Spot14（THRSPα） gene was investigated in Zi geese,Wanxi White geese,Zhedong White geese and Shitou geese. One primer for exon 2 of THRSPα gene were designed according to the homologous sequence of duck. Six SNPs were detected by PCR-SSCP method. Within breeds of four geese were at Hardy-Weinberg equilibrium （P〉0.05）. The results confirmed that there were polymorphisms in the exon 2 of THRSPα gene.

关 键 词：鹅 THRSPΑ基因 PCR-SSCP 

分 类 号：S835[农业科学—畜牧学]