Nulp1基因抑制P19CL6细胞向心肌细胞分化  

Nulp1 Gene Inhibits the P19CL6 Cell Cardiomyocyte Differentiation

作　　者：刘仕杰[1] 梁洁[1] 宋怀婷[1] 邓云[1] 莫小阳[1] 万永奇[1] 李永青[1] 袁婺洲[1] 江志钢[1] 吴秀山[1] 王跃群[1] 

机构地区：[1]湖南师范大学蛋白质化学及发育生物学教育部重点实验室心脏发育研究中心,湖南长沙410081

出　　处：《激光生物学报》2010年第2期246-248,277,共4页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30671053;30871340;30930054);国家重点基础研究发展计划资助项目(2005 CB522505)

摘　　要：Nulp1基因是已克隆的一个新的bHLH转录因子亚家族成员。前期的研究表明:Nulp1蛋白作为一个转录抑制子对SRF信号途径有极强的抑制作用。为了进一步研究Nulp1基因在心肌分化中的作用,在能够高效分化为心肌细胞的P19CL6细胞系中过表达Nulp1基因,发现心肌分化标志基因的表达被抑制;用RNA干扰技术,使Nulp1基因在P19CL6细胞系中的内源表达降低,发现心肌分化标志基因的表达提高,说明Nulp1基因能够抑制P19CL6细胞系向心肌细胞的分化。Nulp1 is a novel human gene had been cloned previously, and the protein product of this gene is a new member of bHLH transcription factor subfamily. Nulp1 is a transcriptional suppressor and can inhibit the transcriptional activity of SRF. P19CL6 cell line can efficiently differentiate into cardiac muscle cells. Overexpression of Nulp1 in P19CL6 cell line strongly inhibited the mark genes expression in the process of cardiac differentiation. Furthermore, the expressions of mark genes were stronger than that of control when silenced Nulp1 by RNAi, indicated that Nulp1 might inhibit the P19CL6 cardiomyocyte differentiation.

关 键 词：P19CL6细胞 心肌分化 Nulp1 RNA干扰 

分 类 号：Q78[生物学—分子生物学]