果蝇心管的拟表型  

Phenocopy in the Fly Heart Tube

作　　者：彭忠禄[1,2] 吴秀山[1] 袁婺洲[1] 

机构地区：[1]湖南师范大学生命科学学院,湖南长沙410081 [2]湘南学院文学化生系,湖南郴州423000

出　　处：《激光生物学报》2010年第2期263-267,F0003,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30570934;30671054);教育部新世纪优秀人才支持计划项目(NCET-05-0713)

摘　　要：基因突变表型的获得是利用果蝇模型进行发育相关基因功能研究的基础。拟表型是指生物体的基因型未发生变化,而由外界环境条件的变化所引起的常与某个特定基因的突变表型相似的表型改变。拟表型的存在往往干扰突变表型的鉴定,为基因功能的研究带来困扰。采用胚胎抗体染色和绿色荧光蛋白GFP心脏特异标记的转基因果蝇(Hand-GAL4;UAS-GFP品系)作为表型检测手段,比较活体胚胎、固定处理胚胎和低温处理幼虫中果蝇心管拟表型产生的概率以及统计分析其方法的差异,结果显示胚胎固定处理可以明显减少拟表型出现的概率,而低温固定幼虫也是有效减少拟表型的方法。拟表型和突变表型必须通过统计分析才能有效区分。To obtain genetic mutant phenotypes is the basic foundation for the functional study of developmental related genes using fly model. Phenocopy is a phenotypic trait that is induced by environmental factors but closely resembles a phenotype usually produced by a specific mutant gene. The existance of phenocopy usually interferes in the identification of mutant phenotype and brings troubles in the study of gene function. Antibody staining of whole mount Drosophila embryos and GFP heart specific marker transgenic flies （ Hand-GAIA ;UAS-GFP line） were used as phenotype test facility to compare phenocopy probability ratio among fly living embryos, fixed embryos and low temperature treated embryos , while statistic analysis of differences in three independent methods was performed in the end. The results indicated that embryos fixing treatment could obviously decrease the probability ratio of phenocopy, though low temperature treatment was also an effective way to decrease phenocopy, phenocopy and mutant phenotype could only be effectively distinguished through statistical analysis of experimental data.

关 键 词：绿色荧光蛋白(GFP) 表型标记 拟表型 

分 类 号：Q343.1[生物学—遗传学]