中华蜜蜂工蜂复眼的胚后发育研究  

Studies on postembryonic development of compound eye in the honey bee(Apis cerana cerana)

作　　者：李兆英[1,2] 奚耕思[2] 

机构地区：[1]陕西教育学院生命科学系,陕西西安710061 [2]陕西师范大学生命科学学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2010年第3期60-64,共5页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省自然科学研究计划项目(2005C125);陕西教育学院科研基金重点项目(08KJ009)

摘　　要：通过形态解剖、免疫组织化学、原位细胞凋亡检测等技术,对中华蜜蜂工蜂复眼的胚后发育过程进行了比较研究.结果表明:中华蜜蜂的复眼产生自幼虫头壳表皮的眼分化中心.在3龄幼虫出现的形态发生沟沿背-腹轴方向扫过整个复眼发生区,其后部细胞聚集成群,最终形成光感受器.细胞的增殖从幼虫早期开始,到末龄幼虫达到高峰;活跃分裂的细胞主要集中在两条形态发生沟外侧的增殖细胞带内;那里的细胞凋亡也非常活跃.中华蜜蜂视觉细胞发出的视神经进入前脑大约是在蛹发育的第5天.A comparative research on the eye developing process of Apis cerana cerana was carried out,based on the techniques of anatomy,immunohistochemistry and in situ cell death detect,and the characteristics of the proliferation and programmed cell death during compound eye growing process. The results are as follows：The adult compound eyes are generated from a group of epithelial cells lying within the larval head capsule. Proliferation of these cells begins the feeding larval stage but accelerates at the end of the final larval instar. Proliferation occurs in two zones of mitotic activity; these zones flank a morphogenetic furrow （MF）. The furrow and flanking mitotic zones migrate from posterior to anterior across the presumptive compound eye epithelium. Posterior to the furrow,presumptive compound eye cells form clusters and extend axons into the larval optic nerve during pupal fifth day. The number of apoptotic nuclei in two zones of mitotic activity gradually increased. The clear coincidence in time and space of both apoptosis and proliferation.

关 键 词：中华蜜蜂 复眼 细胞增殖 细胞凋亡 

分 类 号：Q954.67[生物学—动物学]