Graves病和桥本甲状腺炎患者甲状腺原位凋亡和Fas/FasL的表达  

The determination of apoptosis and expression of Fas /FasL in thyroid cells of AITD

作　　者：陈瑜[1] 刘东方[2] 叶果[1] 

机构地区：[1]重庆市肿瘤研究所内科,重庆400030 [2]重庆医科大学附属第二医院内分泌科,重庆400010

出　　处：《南京医科大学学报（自然科学版）》2010年第5期673-677,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：重庆市自然科学基金(CSTC2006BB5293)

摘　　要：目的:探讨正常人、Graves病(GD)和桥本甲状腺炎(HT)患者甲状腺细胞(TEC)凋亡率的差异,并观察凋亡相关蛋白的表达情况。方法:TUNEL法检测3种甲状腺组织的TEC凋亡率;免疫组化检测TEC凋亡蛋白Fas、FasL表达情况,RT-PCR检测FasmRNA表达的差异。结果:GD和HT患者TEC凋亡率显著高于正常对照(P<0.05),HT患者TEC凋亡率较GD患者也显著升高(P<0.05),FasL在正常、GD和HT甲状腺组织中存在连续性表达,但Fas在自身免疫性甲状腺疾病(AITD)患者的表达水平显著高于正常甲状腺组织,以HT的Fas表达水平最高(HT与正常对照相比,P<0.01;GD与正常对照比,P<0.05)。结论:凋亡可能在AITD的发病中起一定作用,Fas及其配基系统是与AITDTEC凋亡相关的蛋白。Objective：To explore the apoptosis rate of thyroid cells from normal,Graves disease（GD）,Hashimoto thyroiditis（HT） and detect the apoptosis related proteins.Methods：The apoptosis rate of thyroid cell from normal GD and HT thyroid gland was measured by the terminal deoxynucleotidyl transferase mediated d-UTP nick labeling（TUNEL）,and apoptosis related proteins Fas and FasL were confirmed by immunohistochemical methods,and the expression of Fas mRNA was measured by RT-PCR.Results：Higher apoptosis rate was found in GD and HT TEC,and there was an obvious and almost equal expression of FasL（P 〉0.05）.The difference in expression of Fas protein and mRNA in three groups thyroid glands was significant（P 〈0.01）,and the expression of Fas in normal tissue was the lowest and trivial,but the Fas expression in HT was the highest and distinctive.Conclusion：These data indicate that apoptosis plays an important role in the pathogenesis of AITD,and Fas /FasL may be the signal molecule related to apoptosis of TEC.

关 键 词：GRAVES病 桥本甲状腺炎 甲状腺 凋亡 凋亡蛋白 

分 类 号：R581.1[医药卫生—内分泌]