一种膜荚黄芪凝集素的分离纯化研究  

Isolation and purification of a lectin from roots of Astragalus membranaceus

作　　者：朱利芬[1] 闫巧娟[2] 江正强[1] Kumar Narasimha 黄林华[2] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国农业大学工学院,北京100083

出　　处：《中草药》2010年第5期714-717,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(20502032);教育部"新世纪优秀人才支持计划"(NCET-08-0534)

摘　　要：目的从膜荚黄芪根中分离纯化一种凝集素。方法通过硫酸铵分级沉淀和ConA-Sepharose4B亲和色谱从膜荚黄芪根浸提液中分离纯化一种凝集素。结果经过上述两步纯化得到电泳纯的凝集素,SDS-PAGE和Superdex75凝胶过滤色谱测定该凝集素的相对分子质量分别为3.15×104和3.35×104,糖蛋白染色显示其为糖蛋白,总糖的量为10.7%。结论从膜荚黄芪根中分离纯化得到一种凝集素,该蛋白为单亚基糖蛋白,具有对兔血红细胞的凝集活性,凝集活性的比活力为391.9U/mg。Objective To isolate and purify a lectin from the roots of Astragalus membranaceus. Methods The protein was purified using a combination of 20%-60%ammonium sulfate fraction and ConA-Sepharose 4B affinity chromatography. Results The purified protein appeared as a single band with molecular mass of 3.15 × 10^4 on SDS-PAGE and the relative molecular mass was estimated by gel filtration on a calibrated Superdex 75 column with apparent molecular weight of 3.35× 10^4. This lectin was a glyco- protein with a neutral carbohydrate content of 10.7 %. Conclusion A lectin is isolated and purified from the roots of A. membranaceus for the first time. It is a monomer glycoprotein and its specific activity is 391.9 U/mg.

关 键 词：膜荚黄芪 凝集素 纯化 亲和色谱 

分 类 号：R284.2[医药卫生—中药学]