高效毛细管电泳法测定复方苦参结肠溶胶囊中氧化苦参碱  

Determination of oxymatrine in Compound Kushen Colon-specific Capsula by capillary electrophoresis

作　　者：仝战旗[1] 高建义[2] 陈丙跃 

机构地区：[1]解放军总医院南楼中医针灸科,北京100853 [2]中国航天员科研训练中心,北京100094 [3]北京中惠药业有限公司,北京100085

出　　处：《中草药》2010年第5期725-727,共3页Chinese Traditional and Herbal Drugs

基　　金：北京市科委"创新药物研究开发"资助项目(D08080200290805)

摘　　要：目的建立HPCE测定复方苦参结肠溶胶囊中氧化苦参碱的方法。方法选用苯异丙胺硫酸盐为内标,石英毛细管柱(50cm×50μm,有效柱长45cm),30mmol/LpH5.8的磷酸缓冲液,分离电压20kV,紫外检测波长为214nm,柱温(25±1)℃。结果氧化苦参碱测定的线性范围为30～240μg/mL,平均回收率为99.54%,RSD为1.86%。结论该法结果准确,操作简单,重现性好,可作为复方苦参结肠溶胶囊中氧化苦参碱的质量控制方法 。Objective To establish an HPCE method for the determination of oxymatrine in Compound Kushen Colon-specific Capsula. Methods Amphetamine sulfate was used as the internal standard. The separation was obtained with silica capillary column （50 cm ×50 μm, effective length 45 cm）, 30 mmol/ L phosphate buffer （pH 5.8） at a constant voltage of 20 kV, and temperature at （25±1） ℃, the detective wavelength 214 nm. Results The linear determination range was 30-240μg/mL and the average recovery and RSD were 99.54% and 1.85%, respectively. Conclusion The method is simple and accurate, and can be used for quality control of Compoud Kusheng Colon-specific Capsula.

关 键 词：复方苦参结肠溶胶囊 氧化苦参碱 高效毛细管电泳 

分 类 号：R286.02[医药卫生—中药学]