GAP生产基地龙胆的质量控制及评价  

Quality control and evaluation of Radix et Rhizoma Gentianae from GAP production base

作　　者：曹悦[1,2] 左代英[2] 刘敬武 孙启时[2] 

机构地区：[1]辽宁省食品药品检验所,辽宁沈阳110023 [2]沈阳药科大学,辽宁沈阳110016 [3]辽宁天瑞绿色产业科技开发有限公司,辽宁清原113300

出　　处：《中草药》2010年第5期821-825,共5页Chinese Traditional and Herbal Drugs

基　　金：国家科技支持计划(2006DAI06A05-08)

摘　　要：目的建立龙胆药材的指纹图谱研究方法并进行聚类分析。方法色谱柱为Shimadzu C18(3.0mm×75mm,1.7μm);流动相:乙腈-0.4%磷酸(5.5∶94.5),体积流量:0.8mL/min;检测波长:240nm;柱温为40℃。结果獐牙菜苦苷、龙胆苦苷分别在0.1548～1.858mg/mL,0.322～5.152mg/mL呈良好的线性关系,平均回收率分别为102.09%,RSD=1.68%(n=6);103.80%,RSD=1.11%(n=6)。指纹图谱的精密度与重现性试验中的各色谱峰的相对保留时间与相对峰面积的RSD小于2.1%,指纹图谱相似度结果与聚类分析谱系图一致。结论建立的指纹图谱适用于龙胆药材的标准化种植及质量控制。Objective To establish method for the fingerprint of Radix et Rhizoma Gentianae using UFLC with the hierarchical cluster analysisMethods Column： Shimadzu C18（3.0 mm×75 mm, 1.7 μm）;mobile phase： Acetonitrile 0.4% phosphorylation （5.5∶94.5）; flow rate： 0.8 mL/min; detection wavelength： 240 nm; temperature： 40 ℃Results This method had a good linearity in the range of 0.154 8—1.858 mg/mL, and the average recovery was 102.09% with RSD of 1.68% for swertiamarin.It had a good linearity in the range of 0.322—5.152 mg/mL, and the average recovery was 103.80% with RSD of 1.11% for gentiopicroside The RSD of precision and repro ducibility were lower than 2.1%. The result of fingerprint studying was the same with the hierarchical cluster analysis.Conclusion This method can be used as standardized implantation and quality control of Radixet Rhizoma Gentianae.

关 键 词：龙胆 指纹图谱 聚类分析 超高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]