Involvement of pro-inflammatory and anti-inflammatory cytokines in the anti-inflammatory activity of Rubus idaeus L.on LPS-treated RAW 264.7 cells  

覆盆子体外抗炎活性评价(英文)

作　　者：肖飞 朱江 赵雷[3] 郑国华[4] 杨安树[5] 陶君彦[4] 张宝徽[4] 黄志军 熊富良 

机构地区：[1]武汉健民集团,湖北武汉430054 [2]江西省食品工业研究所,江西南昌330029 [3]华中科技大学协和医学院传染科,湖北武汉430022 [4]湖北中医学院药学院,湖北武汉430065 [5]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《Journal of Chinese Pharmaceutical Sciences》2010年第3期201-208,共8页中国药学（英文版）

摘　　要：Anti-inflammatory activity ofRubus idaeus L. and possible mechanisms involved were explored in lipopolysaccharide （LPS）-treated RAW 264.7 cells. The effects of ethanol extract of R. idaeus on the levels of pro-inflammatory cytokines, including tumor necrosis factor-alfa （TNF-α）, interleukin-6 （IL-6） and interleukin-1 beta （IL-1β）, as well as pro-inflammatory mediators, such as nitric oxide （NO）, inducible nitric oxide synthase （iNOS） and cyclooxygenase-2 （COX-2） were studied by Sandwich ELISA, real-time PCR, and Western blot analysis. Moreover, the effects of ethanol extract of R. idaeus on anti-inflammatory cytokine interleukin-10 （IL-10） and anti-inflammatory mediator heme oxygenase-1 （HO-1） were also investigated using the same methods. Furthermore, nuclear factor-gB （NF-κB） level was assayed by immunocytochemistry. The results showed that the production of IL-1β, IL-6, NO, TNF-α and COX-2 in LPS-treated cells could be significantly inhibited （P〈0.01 or P〈0.05） by ethanol extract ofR. idaeus compared with that in the cells treated with LPS only. Meanwhile, the production of NF-r,B was also inhibited by the extract. Based on these results, the anti-inflammatory activity ofR. idaeus was attributed to the down-regulation of IL-6, IL-1β and TNF-α levels as well as gene expression of iNOS and COX-2 through the suppression of NF-κB activation, and induction of anti-inflammatory cytokine IL- 10 and anti-inflammatory mediator HO- 1.本文采用脂多糖处理的RAW 264.7细胞为炎症细胞模型,采用ELISA、实时荧光定量PCR、免疫印迹、免疫细胞化学等方法探讨了覆盆子提取物对炎症发生过程中促炎、抗炎细胞因子及NF-κB的影响。结果表明,覆盆子提取物能够明显抑制脂多糖处理的RAW 264.7细胞中IL-1β、IL-6、NO、TNF-α和COX-2的表达(P<0.01或P<0.05),增强IL-10、HO-1等抗炎细胞因子的表达(p<0.01或P<0.05),并且提取物能够抑制NF-κB的产生。因此,我们推断覆盆子提取物具有较好的抗炎作用,其起效的可能机制是通过抑制NF-κB激活来抑制促炎因子的产生,并促进抗炎因子的表达。

关 键 词：Rubus idaeus L. RAW 204.7 cell NF-κB Pro-inflammatory cytokines Anti-inflammatory cytokines 

分 类 号：R285.5[医药卫生—中药学]