着色性干皮病基因D多态性与慢性苯中毒发病风险  

Genetic polymorphism in XPD related to risks of chronic benzene poisoning

作　　者：李岩[1] 张忠彬[1] 孙品[2] 万俊香[2] 金锡鹏[2] 夏昭林[2] 

机构地区：[1]山东省职业卫生与职业病防治研究院,济南250062 [2]复旦大学公共卫生学院

出　　处：《卫生研究》2010年第3期286-290,294,共6页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30271113);国家973项目(No.2002CB512902)

摘　　要：目的探讨XPD的基因多态性与个体慢性苯中毒发病风险的关系。方法采用病例-对照设计,以152名苯中毒工人为病例组,152名接触苯而没有中毒表现的工人为对照组。应用聚合酶链反应-限制性片断长度多态性分析技术(PCR-RFLP)检测XPDc.199、XPDc.201、XPDc.312和XPDc.751位点的多态性。结果未检测到XPDc.199、XPDc.201位点的突变基因型;与携带XPDc.312Asp/Asp基因型的个体相比,调整性别、工龄和暴露强度后,携带XPDc.312Asp/Asn+Asn/Asn基因型的个体的慢性苯中毒的发病风险降低(ORadj=0.59,95%CI=0.35～0.99,χ2=3.99,P<0.05),在低强度苯接触组,该突变基因型的保护作用更为显著(ORadj=0.15,95%CI=0.04～0.51,χ2=8.93,P<0.01)。结论XPDAsp312Asn基因多态可能与个体慢性苯中毒发病风险的改变有关。Objective To explore the relation between genetic polymorphisms in XPD and risks of chronic benzene poisoning（CBP）.Methods A case-control study was conducted.152 CBP patients and 152 NCBP workers occupationally exposed to benzene were investigated.Polymerase chain reaction-restrained fragment length polymorphism technique（PCR-RFLP） was applied to detect the single nucleotide polymorphisms（SNPs） at c.199,c.201,c.312 and c.751 of XPD gene.Results No variant alleles was detected at c.199 and c.201 of XPD gene.In comparition with the individual genotypes of XPDc.312Asp /Asp,the risk of CBP suffered from the individual genotype of XPDc.312Asp /Asn ＋ Asn /Asn decreased a 0.59 fold（ORadj = 0.59,95% CI = 0.35-0.99,χ2 = 3.99,P 0.05）,when sex,workage and intensity of benzene exposure were adjusted.And in low intensity of benzene exposure group,the risk of CBP suffered from the individual genotypes of XPDc.312Asp /Asn ＋ Asn /Asn more decreased（ORadj = 0.15,95% CI = 0.04 ～ 0.51,χ2 = 8.93,P 0.01）.Conclusion Polymorphism of XPD Asp312Asn could contribute to altered risk of CBP.

关 键 词：苯中毒 着色性干皮病基因D 基因多态性 

分 类 号：R135.12[医药卫生—劳动卫生]