4-甲基伞形酮辛酯试验检测水中沙门菌方法研究  

Detection of salmonella in water by using MUCAP test

作　　者：张伟[1] 孙宗科[1] 鲁波[1] 王友斌[1] 丁培[1] 郑萍[1] 陈西平 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《卫生研究》2010年第3期381-383,共3页Journal of Hygiene Research

基　　金：国家科技支撑计划"十一五"项目(No.2006BAJ02A10)

摘　　要：目的研究4-甲基伞形酮辛酯(MUCAP)试验检测水中沙门菌的灵敏度、特异度和检出限以及该方法的实际应用效果。方法采用"前增菌+选择性增菌+MUCAP初筛+氧化酶实验+血清学实验"的方法,对加标水样和实际环境水样进行检测。结果MUCAP试验的灵敏度为100%,特异度为80.8%,检出限为1cfu/100ml。结论采用MUCAP实验的方法可以有效的检出水样中的沙门菌,并将实验时间由原来的72h缩短为48h。Objective To study the sensitivity,specification,detection limit and the practical use of MUCAP method for the detection of salmonella in water.Methods A comprehensive method of pre-enrichment ＋ selective cultivation ＋ initial screening with MUCAP ＋ oxidase test ＋ serological test was applied to check salmonella in water samples spiked with bacteria and environmental water samples.Results the sensitivity of MUCAP method was 100%,the specification was 80.8%,and the detection limit was 1cfu /100ml.Conclusion MUCAP method can effectively identify salmonella in water and the testing time was shortening from 72h to 48h.

关 键 词：沙门菌 检测 MUCAP试验 

分 类 号：R123.1[医药卫生—环境卫生学] R378.22[医药卫生—公共卫生与预防医学]