苦参碱、氧化苦参碱、槐定碱对巨噬细胞活性及分泌肿瘤坏死因子-α的影响  

Effects of Matrine, Oxymatrine and Sophordine on Activity and TNF-α Secretion of Macrophage RAW264.7

作　　者：刘太华[1] 刘德芳[1] 王骏[1] 汪晓军[1] 罗陈[1] 

机构地区：[1]中国人民解放军成都军区总医院,四川成都610083

出　　处：《中国中医药信息杂志》2010年第3期31-33,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：全军"十一五"资助课题(06MB193)

摘　　要：目的观察苦参碱、氧化苦参碱和槐定碱对巨噬细胞RAW264.7活性及分泌肿瘤坏死因子-α(TNF-α)的影响。方法6孔培养板培养巨噬细胞RAW264.7,不同浓度的苦参碱、氧化苦参碱和槐定碱与巨噬细胞RAW264.7共培养1d,采用MTT法检测不同浓度的苦参碱、氧化苦参碱和槐定碱对巨噬细胞RAW264.7活性的影响;收集培养液,采用ELISA方法检测每份培养液中TNF-α的含量。结果不同浓度的苦参碱、氧化苦参碱和槐定碱对巨噬细胞RAW264.7活性有不同程度的抑制作用,不同浓度的苦参碱、氧化苦参碱和槐定碱对巨噬细胞RAW264.7分泌TNF-α有抑制作用,浓度越大,抑制作用越强,苦参碱对巨噬细胞RAW264.7分泌TNF-α的抑制作用更明显。结论苦参碱、氧化苦参碱和槐定碱能抑制巨噬细胞RAW264.7的活性及TNF-α的分泌,苦参碱的抑制作用更明显。Objective To observe the effects of matrine, oxymatrine and sophordine on activity and TNF-α secretion of macrophage RAW264.7. Methods Macrophage were cultivated in 6 holes board and cultivated 1 day with different concentration matrine, oxymatrine and sophordine. Macrophage activity was measured with MTT and content of TNF-α in collected culture medium was measured with ELISA. Results Macrophage activity and the function of secreting TNF-α was restrained by matrine, oxymatrine and sophordine. The higher concentration of matrine, oxymatrine and sophordine was, the more intense the inhibiting effect was. The effect of matrine was more intense to the function of secreting TNF-α of macrophage. Conclusion Matrine, oxymatrine and sophordine can restrain macrophage activity and the function of secreting TNF-α, the effect of matrine is the most intense.

关 键 词：苦参碱 氧化苦参碱 槐定碱 巨噬细胞 肿瘤坏死因子-Α 银屑病 

分 类 号：R285.5[医药卫生—中药学]