猪链球菌2型新毒力相关基因lin缺失菌株的构建  

Identification of a novel virulence-related gene lin of Streptococcus suis serotype 2

作　　者：李鹏[1,2] 刘军[2] 祝令伟[2] 齐翀[2] 周博[2] 孙洋[2] 纪雪[2] 刘爽[1] 王文东[1] 冯书章[1,2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国兽医学报》2010年第5期643-646,共4页Chinese Journal of Veterinary Science

基　　金：国家"973"计划资助项目(2006CB504402);吉林省科技发展计划资助项目(20080130)

摘　　要：利用温度敏感型穿梭自杀质粒pSET4s,定点敲除猪链球菌2型野生型强毒株ZY458的lin基因,构建基因缺失突变菌株458Δlin,利用家兔感染模型对ZY458及其突变菌株的生物学特性进行比较研究。家兔感染试验表明,接种ZY458菌株的家兔体质量下降,出现明显的临床症状,5只家兔4d内全部死亡;而458Δlin感染组家兔生长正常,未出现任何明显临床症状。结果表明,lin基因是猪链球菌2型新发现的一种毒力相关基因,在猪链球菌2型的致病过程中具有重要作用。To investigate virulence factor in pathogenesis of Streptococcus suis serotype 2,a lin gene deletion mutant 458lin of S.suis serotype 2 wild-type strain ZY458 was constructed by use of suicide vector pSET4s.The virulence of wild-type strain ZY458 and deletion mutant 458lin were assessed by rabbits infection model.Animal assays showed that all rabbits infected with ZY458 developed body weight loss and severe clinical symptoms.All 5 rabbits infected with ZY458 died within 4 days post inoculation.S.suis 2 could be recovered from the liver,brain or kidney of rabbits infected with ZY458.In contrast,all 5 rabbits inoculated with 458lin gained weight normally and did not develop any clinical symptoms during the entire course monitored.S.suis 2 could not be detected from organs of all rabbit infected with 458lin.These observations indicated that lin gene is a novel virulence-related gene that may play an important role in the evolution and pathogenicity of S.suis serotype 2.

关 键 词：猪链球菌2型 lin基因 毒力因子 

分 类 号：S852.61[农业科学—基础兽医学]