槲皮素对SLT-Ⅱe诱导大鼠肠黏膜微血管内皮细胞分泌细胞因子的影响  

Effects of quercetin on the secretion of cytokine in rat intestinal microvascular endothelial cells induced by SLT-Ⅱe

作　　者：周宏超[1,2] 胡格[2] 刘钟杰[3] 穆祥[2] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]北京农学院兽医学(中医药)北京市重点实验室,北京102206 [3]中国农业大学动物医学院,北京100094

出　　处：《中国兽医学报》2010年第5期661-664,689,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30671543);北京市自然科学基金资助项目(6021001);北京市属市管高等学校人才强教计划资助项目(BAHED)

摘　　要：通过检测槲皮素对SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞分泌NO、ET-1、P-选凝素、sICAM-1及TNF-α的浓度变化,探索中药复方主要成分槲皮素对仔猪水肿病的疗效机制。将培养的肠黏膜微血管内皮细胞分为对照组、SLT-Ⅱe组、不同浓度槲皮素处理组等5个组,采用ELISA法测定了培养3、6、9和12h时细胞培养上清液中NO、ET-1、P-选凝素、sICAM-1及TNF-α浓度的变化。结果,10mg/L槲皮素可下调SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞NO、ET-1及sICAM-1的分泌,12h内使NO、ET-1及sICAM-1的分泌浓度接近正常水平;1、5、10mg/L槲皮素不能下调SLT-Ⅱe诱导的大鼠肠黏膜微血管内皮细胞P-选凝素和TNF-α的分泌。结果表明,槲皮素通过抑制SLT-Ⅱe诱导肠黏膜微血管内皮细胞NO、ET-1、sICAM-1的过量分泌,缓解肠道微循环障碍,减轻炎症反应,达到治疗水肿病的目的。Determining the concentration of NO,ET-1,P-selectin,sICAM-1 and TNF-α which were induced by SLT-Ⅱe in intestinal microvascular endothelial cells of rats,to study the functional mechanism of quercetin on edema disease of swine.The cultured RIMECs in vitro are divided into control group,SLT-Ⅱe group,test 1 group,test 2 group and test 3 group.Using ELISA to detect the concentration of NO,ET-1,P-selectin,sICAM-1 and TNF-α in cell supernatant which were cultivated for 3,6,9 and 12 h,respectively.The results showed that 10 mg/L quercetin reduced the secretion of NO,ET-1and sICAM-1 remarkably.The secretion of P-selectin and TNF-α did not reduce by 1,5 and 10 mg/L quercetin.Quercetin could reduce the overfull secretion of NO,ET-1 and sICAM-1 in IMECs of rat,relive instestinal microcirculation obstruction,alleviate the inflammation response and cure edema disease of swine.

关 键 词：SLT-Ⅱe 肠黏膜微血管内皮细胞 NO ET-1 P-选凝素 SICAM-1 TNF-α 槲皮素 

分 类 号：S852.21[农业科学—基础兽医学]