精子介导法制备GHRH转基因猪的研究  

Production of GHRH transgenic pig by sperm-mediated gene transfer

作　　者：丁景华[1] 吴珍芳[1] 习欠云[1] 刘小龙[1] 蔡伟光[1] 孟繁民[1] 束刚[1] 王松波[1] 江青艳[1] 张永亮[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642

出　　处：《中国兽医学报》2010年第5期693-697,共5页Chinese Journal of Veterinary Science

基　　金：新品种培育重大专项基金资助项目(2008zx08006-004);广东省科技厅农业攻关基金资助项目(2008B020100005)

摘　　要：以eGFP为报告基因,生长激素释放激素(GHRH)为目的基因,利用精子介导的基因转移方法,将构建慢病毒载体质粒LV-EGFP-GHRH直接和猪精子孵育,再通过体外人工受精建立GHRH转基因猪模型。经PCR检测,52头后代中发现7头阳性个体,转基因效率为7.42%。本研究探索了应用精子介导的转基因方法将GHRH转移到动物体内,为猪的转基因育种提供了新的依据。The transgenic pigs carrying GHRH gene were produced using the sperm-mediated gene transfer method.Lentiviral vector LV-EGFP-GHRH of GHRH as aim gene and EGFP as report gene was co-cultured with the sperm cells,and then fertilization in vitro was performed.Seven out of fifty-two offspring were found to be transgenic by PCR characterization carrying GHRH gene and the transgenic ratio was 7.42%.

关 键 词：猪 转基因 精子介导 GHRH 

分 类 号：Q78[生物学—分子生物学] S823[农业科学—畜牧学]