牛SLC11A1基因多态性分析  

Analysis of genetic polymorphisms at SLC11A1 gene of cattle

作　　者：吕文发[1] 郭洋[2] 王洪梅[2] 王长法[2] 李秋玲[2] 侯明海[2] 仲跻峰[2] 

机构地区：[1]吉林农业大学,吉林长春130118 [2]山东省农业科学院奶牛研究中心,山东济南250100

出　　处：《中国兽医学报》2010年第5期698-703,共6页Chinese Journal of Veterinary Science

基　　金：国家高技术研究发展计划基金资助项目(2006AA10Z1D9;2007AA10Z169);公益性行业科研专项基金资助项目(ny-hyzx07-036-09);山东省良种工程基金资助项目(2006LZ10-04);山东省科技攻关基金资助项目(2006GG2209011)

摘　　要：本研究采用巢氏PCR、降落PCR技术扩增牛SLC11A1基因的内含子9和11,分别获得777、715bp的片段,利用DNA测序技术对这2片段测序,发现3个新SNPs,分别为内含子9的6067(A/G)、6358(C/T)和内含子11的7809(A/T)。同时利用CRS-PCR和PCR-RFLP方法对这3个SNPs进行基因型分型,分析944头中国荷斯坦牛、鲁西黄牛和渤海黑牛的SLC11A1基因的多态性。结果表明,SLC11A1基因的3个SNPs的AA基因型频率最高,优势等位基因均为A;适合性检验表明,6358(C/T)和7809(A/T)位点在中国荷斯坦牛与鲁西黄牛群体中已达到Hardy-Weinberg平衡状态(P>0.05),而6067(A/G)位点的突变在牛群中均未达到Hardy-Weinberg平衡状态(P<0.05);同时中国荷斯坦牛群体在这3个基因座位上的多态信息含量均大于鲁西黄牛与渤海黑牛。Nest-PCR and touchdown PCR technique were used to amplify 777 bp and 715 bp fragments of intron 9 and 11 of solute carrier family 11 member 1（SLC11A1）gene,respectively.Sequencing results showed that three new single nucleotide polymorphisms （SNPs） were identified at position of 6067（A/G）,6358（C/T） and 7809（A/T） in the SLC11A1 gene intron9 and 10,respectively.The SNPs of SLC11A1 gene in China Holstein cattle,Luxi Yellow cattle and Bohai Black cattle （n=944） were genotyped by CRS-PCR and PCR-PFLP.The results show that three genotypes namely AA,BB,and AB were detected,and Allele A and genotype AA were predominant.Chi-square test indicated that 6358（C/T） and 7809（A/T） of China Holstein and Luxi Yellow were in accordance with the Hardy-Weinberg equilibrium （P0.05）,while 6067（A/G） polymorphic sites did not meet Hardy-Weinberg equilibrium （P0.05） in three different cattle.The value of polymorphism information content of three SNPs in China Holstein cattle was higher than Luxi Yellow cattle and Bohai Black cattle.

关 键 词：牛 SNPS SLC11A1基因多态性 CRS-PCR PCR-RFLP 

分 类 号：S857.2[农业科学—临床兽医学] Q78[农业科学—兽医学]