天津野生白刺再生体系的建立  

Regeneration System of Tianjin Wide N. tangutorum

作　　者：何斌琼[1] 杨海萍[2] 朱文碧[2] 杨静慧[2] 刘艳军[2] 穆俊丽[3] 

机构地区：[1]西南大学园艺学院,重庆400716 [2]天津农学院园艺系,天津300384 [3]西北农林科技大学园艺学院,杨凌陕西712100

出　　处：《北方园艺》2010年第10期159-161,共3页Northern Horticulture

基　　金：天津市自然科学基金资助项目(05YFJMJC14400);天津市科委科技支撑计划资助项目(07ZCKFNC01100;08ZCKFNC01200);天津市农业科技成果转化与推广资助项目(0504018)

摘　　要：以天津野生白刺为材料,通过不同激素、不同外植体、不同含量的MS培养基和赤霉素的筛选试验建立了白刺再生体系。结果表明:以初春时节白刺幼嫩茎段为外植体建立无菌系,以破坏生长点的茎尖为外植体进行再生;最适芽增殖培养基为MS+BA 3.0 mg/L+NAA 0.4 mg/L,最适增粗培养基为1/2MS+BA 1.0 mg/L+0.2 mg/L IAA,最适芽再生培养基是MS+BA 3.0 mg/L+NAA 0.4 mg/L+GA 3.0 mg/L,再生率为94%。最适伸长培养基为MS+BA 1.0 mg/L+IAA0.3 mg/L,最适生根培养基为1/2MS+IBA 0.5 mg/L。The wild N. tangutorum of Tianjin was selected as the material of this experiment. According to the selection experiments of different concentrations of hormone, different explants, contents of MS culture media and gibberellin, the regeneration system was built up. The results showed that the early spring tender stems were the best explants for the asepsis system, and the damaged caudex tip were used as the explant to regenerate. The best multiplication culture medium was MS＋BA 3. 0 mg/L＋NAA 0. 4 mg/L, the best culture medium for aggrandizement was 1/2MS＋BA 1.0 mg/L＋ 0. 2 mg/L IAA,the best culture medium for regeneration was MS＋BA 3. 0 mg/L＋NAA 0.4 mg/L＋GA 3.0 mg/L, the regeneration rate was 94%. The best culture medium for elongation was MS＋BA 1.0 mg/L＋IAA 0. 3 mg/L,and the best culture medium for rooting was 1/2MS＋IBA 0.5 mg/L.

关 键 词：白刺 组织培养 再生 

分 类 号：S793.9[农业科学—林木遗传育种]