The phylogeny of Orthoptera inferred from mtDNA and description of Elimaea cheni(Tettigoniidae:Phaneropterinae)mitogenome  

作　　者：Zhijun Zhou Haiyan Ye Yuan Huang Fuming Shi 

机构地区：[1]a College of Life Science, Hebei University, Baoding 071002, China [2]College of Life Science, Shaanxi Normal University, Xi'an 710062, China

出　　处：《Journal of Genetics and Genomics》2010年第5期315-324,共10页遗传学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.30670279 and 30670252);Talent Foundation of Hebei University(No.2008-138)

摘　　要：The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni （Phaneropterinae） was determined. The putative initiation codon for cox1 was TTA. The phylogeny of Orthoptera based on different mtDNA datasets were analyzed with maximum likelihood （ML） and Bayesian inference （BI）. When all 37 genes （mtDNA） were analyzed simultaneously, the monophyly of Caelifera and Ensifera were recovered in the context of our taxon sampling. The phylogeny of Orthoptera was largely consistent with previous phylogenetic hypotheses. Rhaphidophoridae to be a sister group of Tettigoniidae, and the relationships among four subfamilies of Tettigoniidae were （Phaneropterinae ＋ （Conocephalinae ＋ （Bradyporinae ＋ Tettigoniinae）））. Pyrgomorphidae was the most basal group of Caelifera. The relationships among six acridid subfamilies were （Oedipodinae ＋ （Acridinae ＋ （Gomphocerinae ＋ （Oxyinae ＋ （Callip- taminae ＋ Cyrtacanthacridinae）））））. However, we did not recover a monophyletic Grylloidea. Myrmecophilidae clustered into one clade with Gryllotalpidae instead of with Gryllidae. ML and BI analyses of all protein coding genes （using all nucleotide sequence data or excluding the third codon position, and amino acid sequences） revealed a topology identical to that of the entire mtDNA genome dataset. However, 22 tRNAs genes excluding the DHU loop and TψC loop （TRNA）, and two rRNA genes （RRNA） perform poorly when analyzed as single dataset. Our results suggest that the best phylogenetic inferences were ML and BI methods based on total mtDNA. Excluding tRNA genes, rRNA genes and the third codon position of protein coding genes from dataset and converting nucleotide sequences to amino acid sequences do not positively affect phylogenetic reconstruction.The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni （Phaneropterinae） was determined. The putative initiation codon for cox1 was TTA. The phylogeny of Orthoptera based on different mtDNA datasets were analyzed with maximum likelihood （ML） and Bayesian inference （BI）. When all 37 genes （mtDNA） were analyzed simultaneously, the monophyly of Caelifera and Ensifera were recovered in the context of our taxon sampling. The phylogeny of Orthoptera was largely consistent with previous phylogenetic hypotheses. Rhaphidophoridae to be a sister group of Tettigoniidae, and the relationships among four subfamilies of Tettigoniidae were （Phaneropterinae ＋ （Conocephalinae ＋ （Bradyporinae ＋ Tettigoniinae）））. Pyrgomorphidae was the most basal group of Caelifera. The relationships among six acridid subfamilies were （Oedipodinae ＋ （Acridinae ＋ （Gomphocerinae ＋ （Oxyinae ＋ （Callip- taminae ＋ Cyrtacanthacridinae）））））. However, we did not recover a monophyletic Grylloidea. Myrmecophilidae clustered into one clade with Gryllotalpidae instead of with Gryllidae. ML and BI analyses of all protein coding genes （using all nucleotide sequence data or excluding the third codon position, and amino acid sequences） revealed a topology identical to that of the entire mtDNA genome dataset. However, 22 tRNAs genes excluding the DHU loop and TψC loop （TRNA）, and two rRNA genes （RRNA） perform poorly when analyzed as single dataset. Our results suggest that the best phylogenetic inferences were ML and BI methods based on total mtDNA. Excluding tRNA genes, rRNA genes and the third codon position of protein coding genes from dataset and converting nucleotide sequences to amino acid sequences do not positively affect phylogenetic reconstruction.

关 键 词：MTDNA MITOGENOME Elimaea cheni ORTHOPTERA PHYLOGENY 

分 类 号：Q959.468[生物学—动物学] S816.2[农业科学—饲料科学]