Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes  

作　　者：Fang Kong Cailin Ge Xiaoping Fang Rod J. Snowdon Youping Wang 

机构地区：[1]College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China [2]Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China [3]Institute for Plant Breeding and Crop Science I, Justus Liebig University, Heinrich-Buff-Ring 26-32, Giessen 35392, Germany

出　　处：《Journal of Genetics and Genomics》2010年第5期333-340,共8页遗传学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.30671166 and 30971812);the National Key Project 973(No.2006CB101603)

摘　　要：The diploid species Brassica rapa （genome AA） and B. oleracea （genome CC） were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins （32.0%） and specific proteins （39.8%） of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.The diploid species Brassica rapa （genome AA） and B. oleracea （genome CC） were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins （32.0%） and specific proteins （39.8%） of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.

关 键 词：Brassica rapa Brassica oleracea proteomics molecular marker 

分 类 号：Q78[生物学—分子生物学] S512.101[农业科学—作物学]