斜纹夜蛾核多角体病毒郴州株的形态学、基因组酶切图谱及生物活性测定  

Morphology, Genomic Restriction Endonuclease Analysis and Biological Activity Assay of Spodoptera Litura Nucleopolyhedrovirus Strain in Chenzhou

作　　者：曾维爱 李小一 邓正平 戴陈伟[3] 罗经仁 孙修炼[3] 周玲红 

机构地区：[1]中国烟草中南农业试验站郴州基地,湖南郴州423000 [2]郴州市农业科学研究所,湖南郴州423000 [3]中国科学院武汉病毒研究所,武汉430071

出　　处：《中国烟草科学》2010年第3期41-44,共4页Chinese Tobacco Science

基　　金：湖南省烟草公司科技基金资助项目(09-11Aa30)

摘　　要：对斜纹夜蛾核多角体病毒(SpliNPV)郴州株的多角体形态进行了扫描电镜观察,多角体直径在1.5～2.5μm之间;多角体经切片后透射电镜观察,每个病毒囊膜内包被的核衣壳数为2～7个;提取SpliNPV郴州株和广州株的基因组进行限制性内切酶分析,两者的图谱比较近似;分别以不同浓度的两株SpliNPV病毒悬液感染3龄初斜纹夜蛾幼虫,其半致死浓度分别为1.8×106 OBs/mL和4.2×106 OBs/mL,两者在统计学上没有显著差异。A strain of Spodoptera litura nucleopolyhedrovirus（SpliNPV） isolated in Chenzhou was investigated on morphology,genomic restriction endonuclease analysis and biological activity assay.By using a scanning electron microscope,its polyhedra were found between 1.5-2.5 μm in diameter.By using TEM,it was found each virion particle included 2-7 nucleocapsids.SpliNPV Chenzhou strain genome was analyzed by restriction endonuclease and compared with a Guangzhou strain,they had a similar profile.When assayed on 3r instar of Spodoptera litura larvae,the half-lethal concentration of SpliNPV Chenzhou strain and Guangzhou strain were 1.8×10^6 OBs/mL and 4.2×10^6 OBs/mL,respectively,they were not significantly different each other.

关 键 词：斜纹夜蛾核多角体病毒 电镜观察 基因组酶切 生物活性测定 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治]