MALDI-TOF生物质谱成像技术的进展  

Advances of imaging mass spectrometry based on MALDI-TOF

作　　者：杨帆[1,2] 原剑[1,2] 郑俊杰[1,2] 魏开华[1,2] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]北京蛋白质组研究中心,蛋白质组学国家重点实验室,北京102206

出　　处：《分析仪器》2010年第3期1-8,共8页Analytical Instrumentation

摘　　要：随着MALDI-TOF等生物质谱仪器性能的提升和应用领域的扩大,基于MALDI的质谱成像技术已成为研究肿瘤标志物、药物代谢、脂类分布等方面的有力手段。最新样品前处理方法以及自动化基质喷涂等技术的发展,大大提高了质谱成像技术的灵敏度和分辨率。利用原位酶切技术直接鉴定组织切片上的蛋白质和多肽是IMS当前的热点和关键。体内药物代谢物分布、脂类物质分布以及植物组织的质谱成像等研究逐渐成为新的应用热点。本文较系统地介绍了生物组织质谱成像技术的研究进展。With the improvement of the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOFMS） and the expansion of its application fields, the imaging mass spectrometry （IMS） based on MALDI-TOF has become a powerful tool for studying tumor markers, drug metabolism, lipid distribution and so on. The latest sample pre-treatment methods and auto matrix-coating technologies have greatly increased the sensitivity and resolution of/MS. Direct identification of proteins and peptides on tissues by in-situ enzyme digestion is an important focus and key point of IMS. Distribution of drug metabolites and lipid, and IMS for plant tissues are the new fields of applications. This paper reviews systematically the advances of the research and applications of IMS.

关 键 词：质谱成像 基质喷雾器 原位酶切 福尔马林 三维重建 

分 类 号：R310[医药卫生—基础医学]