超声微泡联合EPO对于急性心肌梗死后大鼠心肌保护作用的研究  

Protective effect of ultrasound targeted microbubbles destruction combined with EPO on rat myocardium after acute myocardial infarction

作　　者：郭庆[1] 任豪[1] 

机构地区：[1]重庆市第九人民医院老年科,400700

出　　处：《重庆医学》2010年第10期1198-1200,共3页Chongqing medicine

摘　　要：目的观察超声微泡联合促红细胞生成素(EPO)对大鼠急性心肌梗死后的心肌保护作用。方法采用结扎冠状动脉前降支的方法建立大鼠心肌梗死模型。将40只成功制作模型的大鼠随机分为超声+微泡造影剂+EPO组(A组)、超声+微泡造影剂组(B组)、单纯EPO组(C组)、单纯手术组(D组)。术后7 d各组随机选取5只大鼠,尾静脉抽血.采用ELISA法检测TNF-α、IL-6的含量。术后28 d心肌组织Ⅷ因子免疫组织化学染色并在显微镜下计数心肌内新生血管和凋亡蛋白Bcl-2、Bax免疫组化染色。采用ELISA检测心肌组织内VEGF蛋白表达情况。结果与其他各组比较,A组TNF-α、IL-6表达降低,新生毛细血管数量最多,为43±4.7条/每高倍视野,Bcl-2表达上调,Bax表达显著下调,VEGF含量为(6.34±2.14)ng/g,差异有统计学意义(P<0.05)。结论超声微泡联合EPO能增强EPO促进毛细血管形成、抑制梗死周围区炎症反应及减少心肌细胞凋亡,从而达到改善急性心肌梗死后心脏功能的作用。Objective To investigate the protective effects of ultrasound targeted microbubbles destruction（UTMD） combined erythropoietin（EPO） on rat myocardium after acute myocardial infarction（AMI）.Methods Rat model of AM1 were built by ligating the left anterior descending coronary artery.40 rats were randomly divided into four groups：UTMD＋EPO treatment group（A）,UTMD only treatment group（B）,EPO only treatment group（C） and surgery only group（D）.On 7 d,serum TNF-α and IL-6 were detected by ELISA.On 28 d,microvessel density（MVD） was counted in high-power field（HPF）under microscope,Bcl-2,Bax were stained by immunohistochemistry.The VEGFexpression in myocardium was detected by ELISA.Results As compared with control group,the levels of TNF-α,IL-6 in treatment group were obviously decreased（P0.05）,the expression of Bcl-2 was increased,while Bax was decreased（P0.05）.The capillary density in the peripheral zone of myocardial infarction was obviously increased（43±4.7/hp）（P0.05）.The content of VEGF in myocardium was（6.34±2.14）ng/g（P0.05）.Conclusion UTMD＋EPO can improve the cardiac function after AMI in rats through the pro-angiogenic,anti-inflammatory,and anti-apoptotic effects.

关 键 词：超声微泡 促红细胞生成素 心肌梗死 

分 类 号：R542.22[医药卫生—心血管疾病]