有机阴离子转运蛋白介导绿原酸肾小管分泌  

Effects of probenecid,taurine and benzylpenicillin sodium on secretion of chlorogenic acid in shuanghuanglian across MDCK monolayer model

作　　者：陈媚[1] 葛建丹[1] 宋必卫[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310032

出　　处：《浙江工业大学学报》2010年第3期322-325,337,共5页Journal of Zhejiang University of Technology

摘　　要：利用狗肾近端小管上皮(MDCK)细胞单层模型比较丙磺舒、牛磺酸、青霉素钠等不同药物转运蛋白底物对双黄连口服液中绿原酸分泌的影响.MDCK细胞以1.5×104个细胞/孔接种于Milli-cell-CMculture plate inserts上培养,定期观察细胞形态,测定跨膜电阻值,待细胞单层达到一定致密程度后进行分泌实验.用M2e酶标仪测定单用双黄连口服液和合用丙磺舒、牛磺酸、青霉素钠时各组双黄连口服液中绿原酸的跨细胞转运情况,计算累积分泌量.实验结果表明,合用不同转运蛋白底物后,双黄连口服液中绿原酸的分泌量均明显降低.可见绿原酸经肾小管上皮细胞的分泌过程是一可饱和过程,有机阴离子转运蛋白部分参与绿原酸的分泌机制.To compare the effects of the drug transporter substrates such as probenecid, taurine and benzylpenicillin sodium on secretion of chlorogenic acid （CGA） in Shuanghuanglian, a compound prescription of traditional Chinese drug, by using MDCK （Madin Darby canine kidney） monolayers model. MDCK cell （1.5× 10^4 cells / holes） was inoculated in Millicell-CM culture plate inserts, the trans-epithelial electrical resistance（TEER） of cell monolayer was detected to ensure the model is available for experiments. We add CGA （in Shuanghuanglian） to the basal lateral compartment and measure and calculate the cumulative secretion amount of CGA in the apical lateral compartment. The secretion amount of CGA is significantly decreased by coadministration of probenecid, taurine or benzylpenicillin sodium. So CGA could be secreted partly via organic anion transporter in the renal tubule epidermal cells.

关 键 词：MDCK细胞模型 绿原酸 有机阴离子转运蛋白 

分 类 号：R285.5[医药卫生—中药学]