高效液相色谱法测定固经丸中黄芩苷含量  

Determination of Baicalin in GUJINGWAN by HPLC

作　　者：张捷[1] 张艳萍[2] 王欢[1] 刘昌叶[1] 谭生建[1] 

机构地区：[1]中国人民解放军第306医院药学部,北京100101 [2]中国人民解放军白求恩军医学院药学系,河北石家庄050000

出　　处：《中国药业》2010年第10期38-39,共2页China Pharmaceuticals

摘　　要：目的建立测定固经丸中黄芩苷含量的高效液相色谱(HPLC)法。方法色谱柱为YWG-NH2分析色谱柱(250mm×4.6mm,5μm),流动相为乙腈-水(26∶74,含5mL三乙胺,磷酸调pH=2.6),流速1.0mL/min,检测波长276nm。结果黄芩苷的理论塔板数为2950,回归方程为Y=796121.5X+621926.0,r=0.9999,进样质量浓度线性范围是20.20～202.00μg/mL。平均加样回收率为100.46%,RSD=2.05%(n=6)。结论该方法操作简便、结果准确,可用于固经丸的质量控制。Objective A quantitative method was developed for the determination of Baicalin in GujingWan by HPLC. Methods Baicalin was chromatographed on a YWG- NH2 column（250 mm ×4.6 nun, 5 μm）using a mixture of acetonitrile and water（26：74, which was adjusted to pH=2.6 by phosphoric acid）, monitored at 276 nm with a flow rate of 1.0mL/min. Results The number of theoretical plates calculated by Baicalin peak was 2 950. The regression equation was Y=796 121.5X＋621 926. 0, r =0. 999 9, which was linear over a range from 20.20-202.0μg/mL. The average recovery was 100.46%, RSD=2.05% （n=6）.Conclusion The method is proved to be sensitive, rapid and accurate,which can be useful to the quality control of GujingWan.

关 键 词：高效液相色谱法 固经丸 黄芩苷 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]