高效液相色谱法测定结石康胶囊中延胡索乙素含量  

Determination of Tetrahydropalmatine in Jieshikang Capsule by HPLC

作　　者：陈琼[1] 石国明 孔泉 梁利香[1] 

机构地区：[1]河南信阳农业高等专科学校,河南信阳464000 [2]河南羚锐制药股份有限公司,河南信阳464000

出　　处：《中国药业》2010年第10期45-46,共2页China Pharmaceuticals

摘　　要：目的建立结石康胶囊中延胡索乙素的含量测定方法。方法采用高效液相色谱法,色谱柱为C18柱(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸(用三乙胺调pH=6.0,35∶65),检测波长283nm。结果该法线性关系良好,平均加样回收率为99.50%,RSD为0.43%(n=6)。结论该法简便、准确、灵敏度高、重现性好,可作为结石康胶囊质量控制方法。Objective To establish a method for the determination of tetrahydropalmatine in Jieshikang Capsule. Methods The HPLC method was developed. The separation was performed on the C18 column（250 mm×4. 6 mm, 5μm）, with acetonitrile -0. 1% phosphoric acid （adjusted to pH = 6.0 with triethylamine, 35 ： 65） as the mobile phase and the detection wavelength at 283 nm. Results The linear relationship was good, the average recovery rate was 99.50%, RSD was 0. 43% （ n = 6）. Conclusion This method is simple, rapid, accurate and sensitive, which can be used for the quality control of Jieshikang Capsule.

关 键 词：结石康胶囊 延胡索乙素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]