一株碱性α-淀粉酶产生菌的筛选及产酶条件优化  

Screening of Alkaline α-Amylase-producing Strain and Condition Optimization

作　　者：宋燕 李津 

机构地区：[1]山东博士伦福瑞达制药有限公司,山东济南250101

出　　处：《食品与药品》2010年第5期167-170,共4页Food and Drug

摘　　要：目的获取工业生产上有潜在应用价值的碱性淀粉酶产生菌。方法土壤中筛选出5株产淀粉酶的菌株;经正交试验确定培养基的最优组成;通过摇瓶发酵初步确定了发酵条件。结果产淀粉酶活性最高的为来源于草地土壤的菌株SⅡ-6,它在发酵8 h时产酶能力最强;优化后的培养基为玉米粉3%,蛋白胨0.8%,磷酸氢二钠0.6%,硫酸铵0.2%,氯化铵0.15%,pH 9.0。于40℃,180 r/min条件下培养,菌株SⅡ-6酶活性可达到2 114 U/mL。结论筛选出5株产淀粉酶的菌株,其中菌株SⅡ-6是1株耐碱性α-淀粉酶产生菌。Objective To obtain alkaline amylase-producing strains which have potential values in the industry. Methods Five amylase-producing strains were selected from soils. The optimal composition of the medium was determined by the orthogonal test. The fermentation conditions were also initially identified in the shake flask fermentation. Results The strain named SII-6, which was isolated from the grasslands soils, had the highest amylase- producing activity when it was cultured for 8 h. The optimal fermentation medium with pH 9.0 was composed of 3 % corn flour, 0.8 % peptone, 0.6 % disodium hydrogen phosphate, 0.2 % ammonium sulfate and 0.15 % ammonium chloride. After cultivated for 8 h in shaker at 40 ℃ with 180 r/min, the amylase-producing activity of SII-6 reached 2114 U/ mL. Conclusion Five amylase-producing strains can be screened out, in which the strain SII-6 is a alkaline-resistant α-amylase-producing strain.

关 键 词：碱性淀粉酶 菌株筛选 正交试验 优化 

分 类 号：Q556.2[生物学—生物化学]