姜黄素对SMMC-7721人肝癌细胞凋亡和细胞周期的影响  

Effects of curcumin on proliferation,cell cycle status and apoptosis of human hepatocellular carcinoma SMMC-7721 cells

作　　者：赵毅辉[1] 李德俊[1] 王顺金[2] 

机构地区：[1]南昌大学研究生院医学部 [2]南昌大学第二附属医院肿瘤科,南昌330006

出　　处：《实验与检验医学》2010年第2期130-133,共4页Experimental and Laboratory Medicine

基　　金：江西省教育厅科学技术研究项目基金资助;编号:GJJ08090

摘　　要：目的探讨姜黄素对体外培养SMMC-7721人肝癌细胞增殖和凋亡的影响。方法选用SMMC-7721人肝癌细胞进行体外培养至对数生长期,以5～160μmol/L姜黄素(5μmol/L、10μmol/L、20μmol/L、40μmol/L、80μmol/L、160μmol/L)分别处理SMMC-7721人肝癌细胞24、48、72h,四甲基偶氮唑蓝(MTT)实验测定细胞的生长抑制率;流式细胞仪检测不同浓度药物对细胞周期及凋亡率的影响。结果 MTT显示姜黄素对SMMC-7721人肝癌细胞增殖抑制率随药物浓度增加及作用时间延长有升高趋势,呈时间剂量依赖性。流式细胞术显示,随着浓度的升高细胞凋亡率提高,但姜黄素对SMMC-7721人肝癌细胞并不发生周期阻滞作用。结论姜黄素对SMMC-7721人肝癌细胞的生长有显著的抑制作用,并能诱导其凋亡,但不产生周期阻滞作用。Objective To investigate the effects of curcumin on proliferation,cell cycle status and apopto-sis of human hepatocellular carcinoma SMMC-7721 cells in vitro.Methods The human hepatocellular carcino-ma SMMC-7721 cells was cultured to logarithm growing period and treated by curcumin with different concen-tration and different time.The inhibition rates of cells were detected by MTT assay;The cell cycle status and the apoptosis rates were tested by flow cytometry.Results MTT method revealed that curcumin could inhibit the proliferation of hepatocellular carcinoma SMMC-7721 cells in a dose-and time-dependent manner.The tumor cell apoptotic rate in curcumin group was significantly higher than that in control group.Furthermore,flow cytom-etry showed that the apoptotic ratio of curcumin group was increased obviously with the adding up of curcumin than that of control group,yet curcumin did not produce cycle arrest.Conclusions Curcumin on human hepato-cellular carcinoma SMMC-7721 cells may obviously inhibit the cells proliferation and induced apoptosis,but it did not produce cycle arrest.

关 键 词：姜黄素 肝癌 凋亡 周期阻滞 

分 类 号：R285.5[医药卫生—中药学] R735.7[医药卫生—中医学]