拟胚体法诱导人胚胎干细胞神经分化的研究  

Study on neural differentiation of human embryonic stem cells byembryoid bodies

作　　者：袁丁[1] 林戈[1] 卢光琇[1] 

机构地区：[1]中南大学生殖与干细胞工程研究所人类干细胞工程研究中心,湖南长沙410078

出　　处：《湖南中医药大学学报》2010年第3期9-11,共3页Journal of Hunan University of Chinese Medicine

基　　金：国家"863计划"基金资助项目(2006AA02A102);"973计划"基金资助项目(2007CB948103)

摘　　要：目的研究胚胎干细胞（human embryonic stem cells,HESC）向神经祖细胞分化时,拟胚体（embryoidbody,EB）大小和碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）对诱导效率的作用。方法采用人胚胎干细胞形成的EB,按照其直径大小分为3组,拟胚体阶段添加或不添加bFGF分组,检测各组的贴壁效率和神经诱导效率。结果直径350~251μm的EB组和EB阶段未添加bFGF组的贴壁率与神经诱导效率高于其他组EB。结论优化EB大小和培养基成分可促进EB的神经诱导分化。Objective To study the influence of the size of embryoid body（EB） and basic fi-broblast growth factor（bFGF） on neural differentiation of human embryonic stem cells（HESC）.Method EB derived HESC were divided into 3 groups according to their diameters,and grouped with or without bFGF addition during EB stage,then,the replating efficiency and neural induc-tion efficiency in all groups were detected.Results EB of the middle size group（d=350-251 μm） and without bFGF addition group had the highest replating efficiency and induction efficiency.Conclusion Optimization of EB size and medium component could help promote neu-ral differentiation.

关 键 词：胚胎干细胞 拟胚体 神经祖细胞 

分 类 号：Q2-33[生物学—细胞生物学]