小鹅瘟病毒荧光PCR检测方法的建立  

Establishment and application of FL-PCR to detect goose parvovirus viruse

作　　者：龙朕[1] 林颖[2] 张桐源[1] 盛明巍[1] 耿庆华[2] 赵玉军[1] 

机构地区：[1]沈阳农业大学,辽宁沈阳110161 [2]沈阳出入境检验检疫局,辽宁沈阳110016

出　　处：《现代畜牧兽医》2010年第5期51-54,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：依据GenBank小鹅瘟病毒(GPV)的NS基因保守区设计一对特异性引物,利用PCR扩增NS区片段,克隆、测序,采取Taqman探针法,构建小鹅瘟病毒的荧光PCR检测方法。结果表明,该方法检测GPV具有很高的特异性和敏感性,可以用于小鹅瘟病毒的检测。One pair of primers specific based on the conservative reigon of GPV NS designed gene sequences in GenBank was designed. NS designed was amplified by PCR, and then cloned and sequenced. FL-PCR was established by using the technology of Taqman probe ,in order to detect Goose parvovirus Viruse. The result showed that the method was of high specificity and could be used to detect GPV rapidly, which has 100 times higher then PeR in sensitivity.

关 键 词：小鹅瘟病毒 Taqman探针法 荧光PCR 

分 类 号：S852.659.2[农业科学—基础兽医学]