An ELISA Based on a Truncated Soluble ORF2 Protein for the Detection of PCV2 Antibodies in Domestic Pigs  

作　　者：Shuang-hui YIN Shun-li YANG Hong TIAN Jin-yan WU You-jun SHANG Xue-peng CAI Xiang-tao LIU 

机构地区：[1]Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou 730046,China

出　　处：《Virologica Sinica》2010年第3期191-198,共8页中国病毒学（英文版）

基　　金：supported by a project from National Key Technology R&D Program in the 11th Fiveyear Plan of China (2006BAD06A12)

摘　　要：Postweaning multisystemic wasting syndrome (PMWS) is an important swine disease that is closely associated with porcine circovirus type 2 (PCV2). The capsid protein (Cap protein) is a major structural protein that has at least three immunoreactive regions, and it can be a suitable candidate antigen for detecting the specific antibodies of a PCV2 infection. In the present study, an indirect enzyme-linked immunosorbent assay (TcELISA) based on a truncated soluble Cap protein produced in Escherichia coli (E.coli) was established and validated for the diagnostic PCV2 antibodies in swine. The TcELISA was validated by comparison with an indirect immunofluorescence assay (IIFA). The diagnostic sensitivity (DSN), specificity (DSP), and accuracy of the TcELISA were 88.6%, 90.7% and 89.4%, respectively. The agreement rate was 89.38% between results obtained with TcELISA and IIFA on 113 field sera. A cross-reactivity assay showed that the method was PCV2-specific by comparison with other sera of viral disease. Therefore ,the TcELISA will be helpful for the development of a reliable serology diagnostic test for large scale detection of PCV2 antibodies and for the evaluation of vaccine against PCV2 in swine.Postweaning multisystemic wasting syndrome （PMWS） is an important swine disease that is closely associated with porcine circovirus type 2 （PCV2）. The capsid protein （Cap protein） is a major structural protein that has at least three immunoreactive regions, and it can be a suitable candidate antigen for detecting the specific antibodies of a PCV2 infection. In the present study, an indirect enzyme-linked immunosorbent assay （TcELISA） based on a truncated soluble Cap protein produced in Escherichia coli （E. coli） was established and validated for the diagnostic PCV2 antibodies in swine. The TcELISA was validated by comparison with an indirect immunofluorescence assay （IIFA）. The diagnostic sensitivity （DSN）, specificity （DSP）, and accuracy of the TcELISA were 88.6%, 90.7% and 89.4%, respectively. The agreement rate was 89.38% between results obtained with TcELISA and IIFA on 113 field sera. A cross-reactivity assay showed that the method was PCV2-specific by comparison with other sera of viral disease. Therefore ,the TcELISA will be helpful for the development of a reliable serology diagnostic test for large scale detection of PCV2 antibodies and for the evaluation of vaccine against PCV2 in swine.

关 键 词：Porcine circovirus type 2 （PCV2） Soluble ORF2 protein ELISA Antibody detection 

分 类 号：S858.28[农业科学—临床兽医学] S852.65[农业科学—兽医学]