粒细胞集落刺激因子骨髓干细胞动员治疗大鼠肝损伤的实验研究  

Effects of bone marrow stem cell mobilization with Granulocyte colony-stimulating factor for the therapy of liver damage in rats

作　　者：卢兴浩[1] 裴雪莲[2] 肖智伟[1] 郑丽华[1] 慕晓玲[2] 

机构地区：[1]石河子大学医学院 [2]石河子大学医学院组织与胚胎学教研室

出　　处：《农垦医学》2009年第6期487-490,共4页Journal of Nongken Medicine

摘　　要：目的:探讨粒细胞集落刺激因子(G-CSF)骨髓干细胞动员对大鼠肝损伤的治疗作用。方法:将大鼠随机分为正常对照组(10只)、造模组(20只)。造模组采用腹腔注射四氯化碳的方法连续4w构建大鼠肝损伤模型,然后随机分为动员组(10只)和模型对照组(10只),动员组皮下注射G-CSF,连续6d。3w后采用检测大鼠肝功能、大鼠肝脏HE染色等方法进行观察。结果:3w后大鼠的肝功能较模型组没有明显恢复。结论:G-CSF骨髓干细胞动员治疗大鼠肝损伤,不能明显改善肝脏功能,也没有加重肝脏的病理变化。Objective： Explore the granulocyte colony-stimulating factor（G-CSF） to mobilize bone marrow stem cells of rat liver damage in rats.Methods：The rats were randomly divided into normal control（n=10） and model group（n=20）.Model uses the method of intraperitoneal injection of carbon tetrachloride for 4 weeks to build rat liver damage model,and then were randomly divided into the mobilization group（n=10） and model control group（n=10）,and mobilization group were injected of the mobilization of group G-CSF subcutaneous for 6 d.After three weeks liver function,rat liver HE staining was observed.Results：After 3 weeks the liver function of mobilization group no significant recovery than the model group.Conclusion：G-CSF to mobilize bone marrow stem cells in rat liver damage can not significantly improve liver function,and there is no increase the pathological changes in liver.

关 键 词：粒细胞集落刺激因子 大鼠肝损伤 治疗 

分 类 号：Q343[生物学—遗传学]