4种细菌芽胞中α/β-SASP分子结构对比分析  

Comparative Structural Analysis of α/β-Small,Acid-Soluble Spore Proteins(SASPs) from Four Bacillus Strains' Spores

作　　者：程立庆[1] 柴雅明[1] 张林[1] 黄国荣[1] 饶中林[1] 熊鸿燕[1] 

机构地区：[1]第三军医大学军事预防医学院军队流行病学教研室,重庆400038

出　　处：《微生物学杂志》2009年第6期10-15,共6页Journal of Microbiology

基　　金：重庆市科委攻关课题(CSTC;2008AC5005)

摘　　要：认识和描述不同细菌芽胞α/β-SASP的分子结构特征,为深入开展以α/β-SASP为靶向修饰的应用技术提供科学依据。运用生物信息学方法和技术,比对分析4种菌株,炭疽芽胞杆菌Ames株、苏云金芽胞杆菌serovar konkukian 97-27株、腊样芽胞杆菌ATCC 10987株、枯草芽胞杆菌168株的α/β-SASP基因及蛋白质一、二、三级结构的异同。基因-ClustalW2;一级结构-ClustalW2和ProtParam tool;二级结构-SOPMA;三级结构-SWISS-MODEL和Swiss-Pdbviewer4.0.1。4种菌株的α/β-SASP基因及蛋白质一、二、三级结构有明显的同源性,炭疽芽胞、苏云金芽胞和腊样芽胞的生物学特征非常相似。在开展细菌芽胞的α/β-SASP基因及生物效应研究时,可以首选苏云金杆菌芽胞或腊样杆菌芽胞作为炭疽杆菌芽胞的试验菌,其次可以选择枯草杆菌芽胞。In order to provide scientific evidence for further developing techniques targeting at α/β-type small,acid-soluble spore proteins（SASPs） by understanding and describing the molecular structural characteristics of α/β-SASPs from four different Bacillus strains＇ spores.Bioinformatics methods and techniques were used to make a comparative analysis of genes,primary structures,secondary structures and tertiary structures of α/β-SASPs from four strains of Bacillus anthracis strains Ames,B.thuringiensis serovar konkukian strain 97-27,B.cereus ATCC 10987,and B.subtilis 168.Tools and softwares employed were： Clustal W2 for genes;Clustal W2 and ProtParam tool for primary structures;SOPMA for secondary structures;SWISS-MODEL and Swiss-Pdbviewer 4.0.1 for tertiary structures.The results showed that genes,primary,secondary,and tertiary structures of α/β-SASPs from four Bacillus strains had significant homology,and the biological characteristics of Bacillus anthracis,Bacillus thuringiensis and Bacillus cereus were very much resembled.Therefore,when doing researches on α/β-SASP gene of bacterial spore and its biological effects,one could first choose Bacillus anthracis,Bacillus thuriengsis,and Bacillus cereus,and secondly Bacillus subtilis.

关 键 词：芽胞 生物信息学 α/β-SASP 

分 类 号：Q939.1[生物学—微生物学]