奶牛乳腺上皮细胞三维培养的形态观察  

THE THREE-DIMENSIONAL CULTURES OF BOVINE MAMMARY EPITHELIAL CELLS

作　　者：王秀美[1] 考桂兰[1] 高爱武[2] 侯先志[1] 高民[3] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,呼和浩特010018 [2]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [3]内蒙古农牧业科学院,呼和浩特010010

出　　处：《内蒙古农业大学学报（自然科学版）》2009年第4期8-14,共7页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：农业部"奶牛产业技术体系"项目子课题(nycytx-02-05)

摘　　要：本研究利用原代培养后纯化的奶牛乳腺上皮细胞,以Matrigel为基质胶原,进行奶牛乳腺上皮细胞的三维培养,细胞接种浓度分别为1×104细胞/ml、1×105细胞/ml和1×106细胞/ml。经过16d培养后,用DAPI对细胞染色,利用荧光显微镜观察细胞生长状况。结果表明,当细胞接种浓度为1×104细胞/ml^1×105细胞/ml时,细胞形成了团块状结构,出现了类似腔状的腺泡形态。The three-dimensional cultures of bovine mammary epithelial cells were conducted with Matrigel.The density of the cells plated in cell culture plates were respectively 1×104 cells/ml,1×105 cells/ml and 1×106 cells/ml.After 16 days culture,the cell were stainned with DAPI and the cell morphology were detected by fluorescence microscope.The results suggested that the cells underwent series growth and formed acinar structures described as mammospheres.

关 键 词：奶牛乳腺上皮细胞 三维培养 基质 

分 类 号：S823.1[农业科学—畜牧学]