应用套式PCR分段扩增犬瘟热病毒融合蛋白的全基因  

Amplifying the Whole Gene Encoding the Fusion Protein of Canine Distemper Virus by Nest RT PCR

作　　者：李金中[1] 夏咸柱[1] 殷震[1] 涂长春[1] 金宁一[1] 

机构地区：[1]解放军农牧大学军事兽医研究所

出　　处：《中国兽医学报》1999年第2期124-125,共2页Chinese Journal of Veterinary Science

基　　金：军队"八五"医药卫生重点课题

摘　　要：为研究犬瘟热病毒（ＣＤＶ）融合蛋白各段功能，根据ＣＤＶＯｎｄｅｒｓｔｅｐｏｏｒｔ弱毒株的融合蛋白基因序列，设计合成了１０条寡聚核苷酸引物。对此１０条引物进行不同的配对，将１株犬瘟热疫苗弱毒株细胞培养物的总ＲＮＡ进行ＲＴ－ＰＣＲ扩增，利用１次ＰＣＲ扩增得到了７个基因片段，最长的达１６６９ｂｐ，最短的为３１４ｂｐ；应用套式或半套式ＰＣＲ。Ten oligonucleotide primers were designed and synthesized according to the nucleotide sequence of the fusion protein gene of the attenuated Onderstepoort strain of canine distemper virus (CDV) to provide the basis for study the function of CDV fusion protein. Seven gene fragments were amplified from one attenuated vaccine strain by reverse transcriptase polymerase chain reaction (RT PCR) in one time with the longest fragment to be of 1 669 bp and the shortest fragment of 314 bp. Eight gene fragments were amplified by nest or half nest PCR which might cover the whole gene encoding the CDV fusion protein.

关 键 词：犬瘟热病毒 融合蛋白基因 RT-PCR 套式PCR 

分 类 号：S852.655[农业科学—基础兽医学] Q78[农业科学—兽医学]