传染性法氏囊病病毒VP2/VP0基因在重组鸡痘病毒中的表达  

Expression of IBDV VP2/VP0 Genes in Recombinant Fowlpox Viruses

作　　者：刘毅[1] 金宁一[1] 郭志儒[1] 方厚华[1] 古长庆 罗坤[1] 李萍 殷震[1] 

机构地区：[1]解放军农牧大学军事兽医研究所

出　　处：《中国兽医学报》1999年第2期126-128,共3页Chinese Journal of Veterinary Science

基　　金：国家863计划资助项目

摘　　要：以鸡痘病毒复制非必需区ＴＫ基因为侧翼，在牛痘病毒Ａ型包涵体晚期启动子（ＡＴＩ）与１６个串联的突变型早期痘苗病毒ｐ７．５启动子组成的复合启动子的下游，分别插入编码传染性法氏囊病病毒（ＩＢＤＶ）结构蛋白ＶＰ２和ＶＰ２－４－３（ＶＰ０）的基因片段后，进行了同源重组和蓝斑筛选，获得２株重组病毒ｖＵＴＡＬａｃＶＰ２－７和ｖＵＴＡＬａｃＶＰ０－１０。经ＥＬＩＳＡ、ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎｂｌｏｔ检测表明，重组病毒ｖＵＴＡＬａｃＶＰ２－７能表达ＶＰ２，ｖＵＴＡＬａｃＶＰ０－１０能表达ＶＰ２和ＶＰ３，ＶＰ２和ＶＰ３的Ｍｒ分别为４１０００和３２０００。Two recombinant plasmids, pUTALac VP2 and pUTALac VP0, were constructed with inserting fragment A VP2 4 3 gene and truncated VP2 gene of IBDV double strand genome at the down stream of hybrid promoter of cowlpox A type inclusion (ATI) promoter. With homologous recombination and screening by X gal, two recombinant viruses, vUTALac VP2 and vUTALac VP0, were obtained. The results of ELISA, SDS PAGE and western blot demonstrated that the two recombinant viruses expressed purpose proteins, 41 000 and 32 000 in size, respectively, which can response specifically to anti IBDV polyclone serum.

关 键 词：传染性 法氏囊病 病毒 IBDV VP2/VP0基因 rFPV 

分 类 号：S855.3[农业科学—临床兽医学] Q78[农业科学—兽医学]