PDGF对瘢痕成纤维细胞影响的动物实验  

The animal experiment of effect PDGF on the fibroblasts of scars

作　　者：袁瑞红[1] 刘流[1] 赵德萍[2] 徐宏慧[1] 孙家平[1] 王福科[1] 刘垠[1] 

机构地区：[1]昆明医学院第一附属医院整形外科,昆明650032 [2]昆明医学院第一附属医院临床医学实验研究中心,昆明650032

出　　处：《中国生物美容》2009年第4期20-23,50,共5页China Bio-beauty

基　　金：云南省自然科学基金资助项目(2005C0072M);云南省社会发展攻关项目(2007CA006)

摘　　要：目的探讨细胞因子PDGF对瘢痕成纤维细胞生物学行为的影响、病理改变,及其对瘢痕的作用机制,为临床预防和治疗瘢痕提供分子及病理学水平的理论依据。方法对大鼠动物创伤模型进行体内实验,采用倒置显微镜、HE染色、Ca son氏改良三色染色、Hamason-Lush-bangh氏染色法、透射电镜等方法进行观察、分析,以研究细胞因子PDGF对瘢痕成纤维细胞生物学行为的影响以及病理改变。结果大鼠动物创伤模型病理染色与透射电镜观察显示,与对照组相比,实验组局部注射细胞因子PDGF后,促进了成纤维细胞的生长增殖,刺激了胶原纤维的合成、增生和沉积。结论细胞因子PDGF对成纤维细胞的生长趋势与增殖活性有明显的促进作用,刺激了胶原纤维的合成、增生和沉积。Objective To explore the mechanism of cytokines for the scars, we study effects PDGF on the fibroblasts of scars in biological behavior and pathological change. It can provide the basic of theory in molecular biology and pathobiology. Materials and Methods Wounds were created on a Sprague-Dawley（SD） rat model. The results were observed and analysed by light inverted microscope（LM）, transmission elextron microscope（TEM）, HE staining, Cason＇s staining and flow cytometry（FCM）. It was determined that effects of PDGF on scars in biological behavior and pathological change. Results In vivo（rat model）：using pathological staining and TEM, compared to the controls, it could be obtained that the fibroblasts, proliferation was enhanced and collagen synthesis was increased by topically applying PDGF. Conclusions PDGF can increase fibroblasts proliferation and collagen synthesis, secretion and accumulation.

关 键 词：瘢痕 细胞因子 成纤维细胞 创伤愈合 

分 类 号：R619.6[医药卫生—外科学]