银杏叶提取物对大鼠视神经挫伤后视网膜形态及视神经GAP-43表达的影响  

Effect of extract of ginkgo biloba on rat retina morphology and GAP-43 expression after optic nerve contusion

作　　者：吴香丽[1] 卞晓芸[1] 王晓庆 

机构地区：[1]河北医科大学第三医院眼科,河北石家庄050051 [2]京天成生物技术(北京)有限公司,北京100085

出　　处：《眼外伤职业眼病杂志》2010年第5期321-324,共4页Journal of Injuries and Occupational Diseases of the Eye with Ophthalmic Surgeries

摘　　要：目的观察银杏叶提取物(EGb761)对大鼠视神经夹挫伤后视网膜形态学及视神经GAP-43表达的影响。方法SD大鼠60只随机分为正常组12只、对照组24只、治疗组24只。后两组建立视神经不完全损伤模型,从伤后1 h开始,隔日1次,对照组给予生理盐水球后注射,治疗组给予EGb761球后注射。在伤后3 d、7 d、14 d、28 d取材,HE染色观察两组视网膜形态学改变;RT-PCR检测两组视神经中GAP-43 mRNA的表达水平。结果各时间点视网膜病理学改变治疗组轻于对照组,GAP-43 mRNA的表达治疗组较对照组高(P<0.05)。结论EGb761可以抑制神经节细胞的损伤,使视神经组织中GAP-43 mRNA表达增加,促进视神经夹挫伤后视神经的再生。Objective Observation of the effect of EGb761 on retinal ganglial cell morphology and growth associated protein-43（GAP-43） in optic nerve that have been contund. Methods 60 SD rats were randomly divided into normal group with 12 rats,control group with 24 rats,treated group with 24 rats.The last two groups make incompletion damage model of optic nerve,1 hour later,gave retrobulbar injection of sodium chloride to the control group,and EGb761 to the treated group every other day.Observation the HE stain of the retina;and detect the GAP-43 mRNA by RT-PCR. Results Through the HE stain,we can see that the pathology demage of retina in treated group is milder than that in the control group.And the treated group can make more expression of GAP-43 mRNA than the control group（P〈0.05）. Conclusion EGb761 can decrease the injury of RGCs,regenerate the optic nerve after injuried,and increase the expression of GAP-43 mRNA in optic nerve.

关 键 词：EGB761 视神经夹挫伤 球后注射 GAP-43 

分 类 号：R285.5[医药卫生—中药学]