威灵仙类药材HPLC指纹图谱鉴别研究  

Identification of clematis raw drugs by HPLC fingerprint

作　　者：王祥培[1] 黄婕[1] 靳凤云[1] 孙宜春[1] 吴红梅[1] 许士娜[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《中成药》2010年第5期704-708,共5页Chinese Traditional Patent Medicine

基　　金：贵阳中医学院博士基金国家中医药管理局"西部"重点学科建设资助项目(200414)

摘　　要：目的:建立柱果铁线莲的高效液相色谱(HPLC)指纹图谱并与流通的威灵仙药材相比较。方法:基于10批柱果铁线莲,采用RP-HPLC法,以Diamonsil-C18(250mm×4.6mm,5μm)色谱柱为分析柱,流动相为乙腈-0.05%磷酸水,采用梯度洗脱,流速0.8mL/min,210nm波长下对不同来源的药材进行检测。结果:建立了柱果铁线莲药材HPLC-UV指纹图谱共有模式,23个共有峰被标定。并对不同来源的威灵仙类药材的HPLC指纹图谱进行相似度比较,结果具有明显差异。结论:HPLC指纹图谱具有方法简便,重复性好,特征性强等特点,可用于威灵仙类药材的鉴别。AIM：To establish HPLC fingerprint for the root and rhizome of Clematis uncinata Champ and to compare the differences of clematis and Clematis uncinata in fingerprint. METHODS：Based on 10 batches of Clematis unciuata Champ,its chromatographic seperation was performed on Diamonsil C18 （250 mm × 4. 6 mm,5 μm）with a mobile phase consisting of acetonitrile -0.05% phosphoric acid,gradient eluent,at the flow rate of 0. 8 mL/min. The UV detection was set at 210 nm. RESULTS：The mutual mode to HPLC-UV fingerprints was set up,and the 23 mutual peaks were indicated. The similarities were compared among Cleuatis uncinata Champ and substitutes collected from different sources there were apparent difference in fingerprint. CONCLUSION：The method is stable and reliable with a good reproducibility and provides a reference standard for identifying medicinal clematis.

关 键 词：柱果铁线莲 威灵仙类药材 HPLC 指纹图谱 鉴别 

分 类 号：R284.1[医药卫生—中药学]