重组日本血吸虫中国大陆株28ku GST免疫刺激复合物对小白鼠的免疫试验  

Vaccination of mice with ISCOM containing recombinant 28 ku GST of Schistosoma japonicum

作　　者：刘金明[1] 叶萍[1] 傅志强[1] 蔡学忠[1] 石耀军[1] 陈永军[1] 林矫矫[1] 王美[1] 林邦发[1] 施福恢[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所

出　　处：《中国兽医科技》1999年第3期10-11,共2页Chinese Journal of Veterinary Science and Technology

基　　金：国家863生物高技术项目;中国农业科学院"九五"重点课题

摘　　要：将体外重组的日本血吸虫中国大陆株２８ｋｕ谷胱甘肽Ｓ转移酶（ｒＳｊ２８ＧＳＴ）与免疫刺激复合物（ＩＳＣＯＭ）结合制备了ｒＳｊ２８ＧＳＴＩＳＣＯＭ。用含８５μｇｒＳｊ２８ＧＳＴ的ｒＳｊ２８ＧＳＴＩＳＣＯＭ及１００μｇｒＳｊ２８ＧＳＴ分别免疫ＢＡＬＢ／ｃ小白鼠，用常规ＥＬＩＳＡ检测体液免疫水平，以日本血吸虫中国大陆株尾蚴攻击后的减虫率表示免疫保护力。结果ｒＳｊ２８ＧＳＴＩＳＣＯＭ免疫组的减虫率为２５．５％，其荷虫数和空白对照组荷虫数相比有显著差异，而ｒＳｊ２８ＧＳＴ免疫组的减虫率仅为１５．２％，与空白对照组相比差异不显著；ＥＬＩＳＡ检测结果，ｒＳｊ２８ＧＳＴＩＳＣＯＭ免疫组比ｒＳｊ２８ＧＳＴ免疫组不仅抗体水平高。The in vitro recombinant 28 ku Glutathione st transferase of schistosoma japonicum (rSj28 GST) was combined with immune stimulative complex (ISCOM) for preparing rSj28 GST ISCOM. Balb/c mice were immunized with rSj28 GST ISCOM containing 85 μg rSj28 GST and 100 μg rSj28 GST, respectively, and then the humoral immunity efficacies were measured by a routine ELISA, and the immune protections were represented with S.japonicum cercariae. The test results revealed that the worm reduction rate of mice immunized with rSj28 GST ISCOM was 25.2%, which was significantly higher than that of mice immunized with rSj28 GST. And the former's antibody levels were also much higher than that of latters' (15.2%), and persisted for a longer interval.

关 键 词：日本血吸虫 小白鼠 ISCOM rSj28GST 

分 类 号：S858.927.3[农业科学—临床兽医学]