力竭运动应激对小鼠T淋巴细胞钾离子通道Kv1.3的调节作用  

Functions of exhausting exercise stress in regulating the potassium ion channels (Kv1.3) of T lymphocytes of mice

作　　者：傅涛[1] 王丽春[2] 郝选明[1] 

机构地区：[1]华南师范大学体育科学学院,广东广州510006 [2]中国医学科学院中国协和医科大学医学生物学研究所病毒免疫室,云南昆明650118

出　　处：《体育学刊》2010年第5期99-103,共5页Journal of Physical Education

基　　金：国家科技支撑计划课题(2006BAK12B02)

摘　　要：为了观察力竭运动应激后对小鼠外周血T淋巴细胞钾离子通道表达以及对细胞调节性容积减少(regulatory volume decrease,RVD)的影响,探讨力竭运动应激导致的免疫抑制的可能机制。复制小鼠力竭模型后从外周血中分离出T淋巴细胞,测定其淋巴细胞转化功能,采用RT-PCR、Western blot分别从基因和蛋白质水平揭示力竭运动应激对小鼠外周血T细胞Kv1.3表达的调节作用,采用细胞成像系统测定T淋巴细胞在低渗透压时细胞容积的变化。结果显示力竭运动应激小鼠T淋巴细胞功能受抑制(P<0.05),小鼠外周血T淋巴细胞Kv1.3 mRNA和蛋白质表达水平明显下调(P<0.05),力竭运动应激会影响RVD的调节能力。结果说明,力竭运动应激导致小鼠外周血T淋巴细胞Kv1.3 mRNA和蛋白质表达水平明显下调,RVD调节能力下降,提示力竭运动应激条件下免疫功能的抑制可能与钾通道有关。In order to observe the effects of exhausting exercise stress on the expression of the potassium ion channels of T lymphocytes in the peripheral blood of mice and the regulatory volume decrease（RVD） of cells,and to probe into a possible mechanism of immunosuppression caused by exhausting exercise stress,the authors separated T lymphocytes from the peripheral blood after having duplicated the mouse exhausting model,measured its lym-phocyte transformation functions,revealed the functions of exhausting exercise stress in regulating the expression of Kv1.3 of T lymphocytes in the peripheral blood of mice from the change of the levels of genes and proteins respec-tively by using RT-PCR and Western blot,measured the change of T lymphocyte volume under a hypotonic condi-tion by using a cell imaging system,and revealed the following findings： exhausting exercise stress suppressed the functions of T lymphocytes of mice（P0.05）;the Kv1.3 mRNA and protein expression level of T lymphocytes in the peripheral blood of mice were regulated down significantly（P0.05）;exhausting exercise stress would affect the regulating ability of RVD.The said findings indicated that exhausting exercise stress caused the significant decrease of the KV1.3 mRNA and protein expression level of T lymphocytes in the peripheral blood of mice,and the weakening of the regulating ability of RVD,which suggested that the suppression of immune functions under an exhausting exercise stress condition may be related to the potassium channels.

关 键 词：运动生物化学 Kv1.3免疫抑制 力竭运动应激 

分 类 号：G804.7[文化科学—运动人体科学]