Ca^(2+)对水分胁迫下小麦诱导蛋白产生的影响  

Effect of Ca 2+ on Induced Protein of Wheat Under Water Stress

作　　者：俞嘉宁[1,2] 高俊凤[1,2] 崔四平 魏建昆[1,2] 

机构地区：[1]西北农业大学基础科学系 [2]河北省农林科学院理化所抗性生理室

出　　处：《干旱地区农业研究》1999年第1期72-77,共6页Agricultural Research in the Arid Areas

基　　金：农业部"八五"应用基础研究项目

摘　　要：水分胁迫及ＡＢＡ处理可诱导丰抗８号小麦幼苗及其悬浮培养细胞中４４．２ＫＤ蛋白亚基的产生或大量合成。高浓度的Ｃａ２＋通道阻塞剂（Ｖｅｒａｐａｍｉｌ）处理丰抗８号小麦幼苗，可明显抑制水分胁迫诱导产生的４４．２ＫＤ蛋白亚基的大量合成，而悬浮培养细胞中，２０μＭ的异博定处理就可显著抑制由ＡＢＡ及ＡＢＡ＋ＰＥＧ所诱导的蛋白亚基含量的提高，说明Ｃａ２＋可能参与了干旱信号在胞内的传递过程；细胞对Ｃａ２＋通道阻塞剂的反应比幼苗更显著、更敏感。Production or mass synthesis of 44.2 KD subunit in wheat seedlings and suspended culture cell of Feng Kang 8 was induced by water stress or ABA. Synthetic of 44.2 KD subunit in seedling of Feng Kang 8 was remarkably inhibited by highly concentrated Verapamil of Ca 2+ . The increase of 44.2 KD subunit content induced by ABA and ABA+PEG in suspension culture was obviously restrained by 20 μm verapamil. It demonstrated that Ca 2+ might involved in drought singal transmitting in cell, moreover the reaction of cell to ca 2+ passageway inhibitor was more remarkable and more sensitive than that of seedling.

关 键 词：冬小麦 水分胁迫 干旱诱导蛋白 CA^2+ 

分 类 号：Q945.17[生物学—植物学] S512.1[农业科学—作物学]