放射免疫分析法比较奶牛粪便和乳汁中的孕酮含量  

Monitoring of fecal progesterone in dairy cows by radioimmunoassay.

作　　者：王旻子 吴美文[1] 徐步进[1] 

机构地区：[1]浙江大学原子核农业科学研究所

出　　处：《浙江农业大学学报》1999年第2期195-197,共3页

摘　　要：利用放射免疫分析方法（ＲＩＡ），以奶样为对照，测定了奶牛粪便中的孕酮（Ｐ４）．用９０％乙醇抽提粪便孕酮，回收率为（９５．２±０．５０）％．通过测定正常发情奶牛、配种后怀孕奶牛和卵巢机能性疾病奶牛粪样和奶样孕酮含量的变化，发现两者趋势一致，有较好的相关性（ｒ＝０．７９８４），而且通过粪样测得的发情周期天数与奶样完全一致（２０．０±１．４１）ｄ．The objective of this study was focused on the effectiveness of progesterone (P 4) in dairy cow feces comparing with those in milk by radioimmunoassay(RIA). 90% ethanol was used to extract fecal progesterone. 3H P 4 was incorporated with dry fecal samples and its recovery was (95.2±0.50)%. Dairy cows of normal estrous cycles ( n =5), pregnancy ( n =1) and dysfunctional ovaries ( n =2) were studied and similar changing profiles of P 4 concentrations in feces and milk were observed ( r =0.798 4). The number of estrous cycle days measured in feces was absolutely coincide with that in milk (20.0±1.41)d. Monitoring of fecal steroids should be emphasized in the reproduction of wild animals and zoo animals.

关 键 词：RIA 孕酮 粪便 奶牛 

分 类 号：S823.3[农业科学—畜牧学]