荧光分析技术在单个生物分子行为及实时检测中的应用  

The Application of Technology of the Fluorescence Aalysis in Action and Real-time Observation of the Single Biological Molecule

作　　者：张岩文[1] 史芹[1] 樊俊[1] 

机构地区：[1]石河子大学师范学院\兵团教育学院,新疆石河子832000

出　　处：《兵团教育学院学报》2010年第2期45-49,共5页Journal of Bingtuan Education Institute

摘　　要：活细胞单分子行为及实时检测研究是生命科学向微观世界深入探索的一个重要标志,它需要生物、化学、物理等多学科的交叉,已经成为目前研究的一个热点。该领域主要研究内容有细胞内单个大分子的实时研究、细胞内大分子的超微量实时检测。文章介绍了近年来对活细胞单分子行为及实时检测研究的几种荧光分析技术及其应用,涉及到的荧光分析技术有全内反射荧光显微术、荧光共振能量转移术、多光子荧光成像术、荧光相关光谱、光漂白后荧光恢复技术、荧光寿命成像显微术、单分子荧光偏振等。这些荧光分析技术以其快速、无损、时空分辨率高的特点,成为了研究单个生物分子行为的重要手段。The single molecule action and real -time observation about the living cells is the important symbol to quest deeply the microcosmic world. It has been a focus of study at present, which is interdisciplinary of biology, chemistry and physics. The main project is real - time research and detection of single molecule in cell in this field. The single molecule action and the application of technology of the fluorescence analysis real - time observation about the living cells are introduced in this paper, which include TIRFM, FRET, RIRFM, MFI, FCS, FLIP, FLIM and smFP. These technologies of the fluorescence analysis have been important means to the single molecule action because of its fast, without damage and accurate distinguish ability of space - time.

关 键 词：荧光分析技术 单个生物分子行为 高分辨率 活体检测 

分 类 号：G02[文化科学]