围绝经期综合征肝气郁结证患者雌激素受体β基因多态性分析  

Estrogen receptor β (ERβ) gene polymorphism of perimenopausal syndrome patients with liver qi stagnation syndrome (LQSS)

作　　者：李红[1,2] 任林[2] 李灿东[1] 林雪娟[1] 

机构地区：[1]福建中医药大学中医证研究基地,福州350108 [2]福建省立医院中医科,福州350001

出　　处：《中华中医药杂志》2010年第6期830-832,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.30772697);福建省中医药科研项目计划重点课题(No.wzzz0904);福建省科技厅青年人才项目(No.2008F3055)~~

摘　　要：目的:探讨雌激素受体β基因(ERβ)多态性(rs3020444)与围绝经期综合征肝气郁结证(气郁证)的关系。方法:选择围绝经期综合征气郁证患者100例、非气郁证86例和正常对照组100例,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测ERβ-T/C多态性。结果:卡方分割显示气郁证组的TT频率(84.0%)高于与非气郁证组(68.6%)及对照组(68.0%),差异显著(P=0.004);气郁证组T等位基因频率(91.0%)高于与非气郁证组(83.1%)及对照组(82.5%),差异显著(P=0.008);Logistic回归分析,以非气郁证组为参照系,调整混杂因素后TT基因型患气郁证的相对危险度为2.222(95%可信区间:1.172-4.744,P=0.015)。结论:ERβ-TT基因型可能是围绝经期综合征气郁证发病的易感因素之一。Objective：To investigate the association between polymorphisms of estrogen receptor β （ERβ） gene （rs3020444） and perimenopausal syndrome （PS） in patients with liver qi stagnation syndrome （LQSS）. Metheds：The ERβT/C genotype in 100 patients of PS of LQSS type （PS-LQSS group）,86 patients of PS of non-LSDS type （PS-non-LQSS group） and 100 healthy subjects （control） was determined by polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） assay. Results：Partitions of χ^2 method showed that the higher prevalence of ERβ-TT genotypes was noted in PS-LQSS group （χ^2=8.307,P=0.004）,and the higher prevalence of T alleles was noted in PS-LQSS group （χ^2=7.129,P=0.008）. and Multinomial logistic-regression indicated that the odds ratios （OR） of the ERβ-TT genotypes vs TC/CC for PS-LQSS was 2.222 （95%CI：1.172-4.744,P=0.015） after adjusting for common miscellaneous factors. Conclusions：It was suggested that ERβ-TT genotypes was significantly associated with PS-LQSS,and ESRβ-TT may be one of the genes that contribute to PS-LQSS.

关 键 词：围绝经期综合征 雌激素受体Β 基因多态性 气郁证 

分 类 号：R271.9[医药卫生—中西医结合]